ELEVATED HEPATIC MITOCHONDRIAL AND PEROXISOMAL OXIDATIVE CAPACITIES IN FED AND STARVED ADULT OBESE (OB OB) MICE
ELEVATED HEPATIC MITOCHONDRIAL AND PEROXISOMAL OXIDATIVE CAPACITIES IN FED AND STARVED ADULT OBESE (OB OB) MICE
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DOI：
10.1042/bj2310439
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发表时间：
1985-01-01
影响因子：
4.1
通讯作者：
HOPPEL, CL
中科院分区：
文献类型：
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作者：
BRADY, LJ;BRADY, PS;HOPPEL, CL
在喂养或饥饿24或48 h的幼年（4-5周龄）和成年（6-9月龄）瘦型和肥胖型ob/ob小鼠中研究了肝脏线粒体和过氧化物酶体的氧化能力。成年肥胖小鼠表现出脂质和非脂质底物的线粒体氧化能力升高（每毫克蛋白质消耗的O原子/分钟），丙酮酸盐+苹果酸除外，柠檬酸合酶和总肉毒碱棕榈酰转移酶活性升高。氧化速率和酶活性不受饥饿和总肉毒碱棕榈酰转移酶。氧化率和酶活性不受饥饿瘦或肥胖小鼠，男性和女性的反应相似。过氧化物酶体棕榈酰辅酶A氧化（nmol/min每毫克过氧化物酶体蛋白）也增加了成年肥胖小鼠的肝脏，并没有改变饥饿。在年轻小鼠中，瘦小鼠和肥胖小鼠的肝脏线粒体和过氧化物酶体氧化能力相当。肥胖ob/ob小鼠线粒体和过氧化物酶体氧化能力的增加似乎随着成熟而发展。
Hepatic mitochondrial and peroxisomal oxidative capacities were studied in young (4-5 weeks old) and adult (6-9 months old) lean and obese ob/ob mice that were fed or starved for 24 or 48 h. The adult obese mice showed elevated capacity for mitochondrial oxidation (ng-atoms of O consumed/min per mg of protein) of lipid and non-lipid substrates, with the exception of pyruvate + malate, and elevated activities of citrate synthase and total carnitine palmitoyltransferase. Oxidative rates and enzyme activities were not affected by starvation and total carnitine palmitoyltransferase. Oxidative rates and enzyme activities were not affected by starvation of lean or obese mice, and both males and females responded similarly. Peroxisomal palmitoyl-CoA oxidation (nmol/min per mg of peroxisomal protein) was also increased in livers of adult obese mice and did not change with starvation. In young mice, hepatic mitochondrial and peroxisomal oxidative capacities in lean and obese mice were comparable. The increased mitochondrial and peroxisomal oxidative capacities appear to develop with maturation in obese ob/ob mice.