SEROLOGIC CROSS-REACTIONS AMONG EHRLICHIA-EQUI, EHRLICHIA-PHAGOCYTOPHILA, AND HUMAN GRANULOCYTIC EHRLICHIA
SEROLOGIC CROSS-REACTIONS AMONG EHRLICHIA-EQUI, EHRLICHIA-PHAGOCYTOPHILA, AND HUMAN GRANULOCYTIC EHRLICHIA
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DOI：
10.1128/jcm.33.5.1098-1103.1995
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发表时间：
1995-05-01
影响因子：
9.4
通讯作者：
MADIGAN, JE
中科院分区：
文献类型：
--
作者：
DUMLER, JS;ASANOVICH, KM;MADIGAN, JE
16SrDNA同源性分析表明，人粒细胞埃立克体病（HGE）病原体与兽医病原体马埃立克体（Erlichia equi）和嗜吞噬细胞埃立克体（Erlichia phagocytophila）密切相关。HGE后，患者产生与大肠杆菌反应的抗体。equi和E.因此，我们假设这些物种密切相关，并具有显著的抗原性。用间接荧光抗体试验（IFA）检测人、马、狗和牛的抗血清中与E.用免疫印迹法鉴定与马、马等埃立克体的特异性反应。等。所有来自HGE患者和感染或免疫dth E的动物的恢复期血清。equi或E.嗜吞噬细胞的E.马Ehrlichia chaffeensis血清与马Ehrlichia chaffeensis血清均无反应，只有1匹马自然感染E.马具有针对森氏埃立克体的血清学反应。用其它埃立克体、立克次体和巴尔通体感染或免疫后获得的人和动物血清与E.通过IFA。e.马免疫印迹显示马抗E抗体有19条带。马血清所有HGE特工，E。equi和E.嗜吞噬细胞的抗血清与E.马，而其他抗埃立克体属物种（Ehrlichia spp.）血清很少或根本不与该抗原反应。HGE试剂、E. equi和E.嗜吞噬细胞的抗血清，而不是其他血清也偶尔与25-，42-，和100-MDa抗原反应。大多数血清与大约56和75 kDa之间的抗原反应，可能是热休克蛋白。HGE代理人E. equi和E. IPA和免疫印迹显示，嗜吞噬细胞菌具有明显的抗原性。结合16S rRNA基因的核苷酸序列，这些数据表明E. equi，马唐E.嗜吞噬细胞埃立克体和人粒细胞埃立克体是密切相关或相同的物种。
Homology in the 16S rDNAs shows that the agent of human granulocytic ehrlichiosis (HGE) is closely related to the veterinary pathogens Erlichia equi and Erlichia phagocytophila. After HGE, patients develop antibodies reactive with E. equi and E. phagocytophila; thus, we hypothesized that these species are closely related and share significant antigenicity. Antisera from humans, horses, dogs, and cattle were tested by indirect fluorescent-antibody assay (IFA) for antibodies reactive with E. equi and other ehrlichiae and tested by immunoblot to identify the specific reactions with E. equi. All convalescent-phase sera from human patients with HGE and from animals infected or immunized dth E. equi or E. phagocytophila had antibodies reactive with E. equi by IFA; no reactions with Ehrlichia chaffeensis occurred with these sera, and only one horse naturally infected with E. equi had a serologic reaction against Ehrlichia sennetsu. Human and animal sera obtained after infection or immunization with other Ehrlichia, Rickettsia, and Bartonella species did not react with E. equi by IFA. E. equi immunoblots revealed as many as 19 bands with equine anti-E. equi serum. All HGE agent, E. equi, and E. phagocytophila antisera tested reacted with a 44-kDa antigen of E. equi, while other anti-Ehrlichia spp. sera reacted with this antigen rarely or not at all. HGE agent, E. equi, and E. phagocytophila antisera but not other sera also reacted occasionally with 25-, 42-, and 100-MDa antigens. Most sera reacted with antigens between approximately 56 and 75 kDa, probably heat shock proteins. The HGE agent, E. equi, and E. phagocytophila share significant antigenicity by IPA and immunoblot. Coupled with the nearly identical nucleotide sequences of 16S rRNA genes, these data indicate that E. equi, E. phagocytophila, and the human granulocytic ehrlichia are closely related or identical species.