Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis
Virulence-associated genes in Escherichia coli isolates from poultry with colibacillosis
复制标题
DOI：
10.1016/s0378-1135(03)00076-2
复制
发表时间：
2003-07-01
影响因子：
3.3
通讯作者：
Vidotto, MC
中科院分区：
文献类型：
--
作者：
Delicato, ER;de Brito, BG;Vidotto, MC
禽致病性大肠杆菌是大肠杆菌病的病原体，它含有几种假定的毒力基因。在这项研究中，我们用聚合酶链反应（PCR）检测了来自我们地区的200株大肠杆菌分离株中16个这些基因的存在。7个毒力基因iutA、iss、cvaC、tsh、papC、papG和felA在大肠杆菌病分离株中的检出率明显高于在健康禽类粪便分离株中的检出率，从而证实了它们在大肠杆菌病中的普遍存在和可能的致病作用。然而，在许多大肠杆菌分离株中未检测到其中的一些基因，27.5%的分离株中未检测到这些基因，这表明应该寻找这些基因的变异和尚未检测到的毒力因素。(C) 2003 Elsevier Science B.V.版权所有
Avian pathogenic Escherichia coli, the causative agent of colibacillosis, harbors several putative virulence genes. in this study we examined by polymerase chain reaction (PCR) the presence of 16 of those genes in 200 colibacillosis isolates from our region. The seven virulence genes iutA, iss, cvaC, tsh, papC, papG and felA were detected significantly more often amongst colibacillosis isolates than in fecal isolates from healthy birds, thereby confirming their worldwide occurrence and possible pathogenic role in colibacillosis. However, several of those genes were not detected in many colibacillosis isolates, and none of them were detected in 27.5% of those isolates, which suggests that variants of those genes and yet undetected virulence factors should be searched for. (C) 2003 Elsevier Science B.V. All rights reserved.