A parylene lift-off process with microfluidic channels for selective protein patterning
A parylene lift-off process with microfluidic channels for selective protein patterning
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DOI：
10.1088/0960-1317/17/3/011
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发表时间：
2007-03-01
影响因子：
2.3
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Atsuta, Kyoko;Suzuki, Hiroaki;Takeuchi, Shoji
本文描述了一种利用图案化的对二甲苯薄膜和PDMS微通道对蛋白质进行高分辨率和选择性图案化的方法。含有所需材料的溶液通过微通道输送到图案点，在图案点上以所需图案穿孔对二甲苯薄膜。蛋白质固定在斑点上后，在平面衬底上形成图案，随后PDMS层和对二甲苯层都被剥离。利用这种方法，获得了间隔2微米的20微米×20微米斑点阵列中的牛血清白蛋白(BSA)的精细图案。还展示了多个荧光珠的选择性图案化。这种方法可以在潮湿的条件下进行，这防止了蛋白质的干燥，并且对于选择性的蛋白质构型非常有用，在不需要的区域上非特异性结合最少。
This paper describes a method for high resolution and selective patterning of proteins using a patterned parylene film and PDMS microchannels. Solutions containing desired materials are delivered through the microchannels to the patterning spots where the parylene film is perforated in a desired pattern. Proteins are patterned on a planar substrate after being immobilized on the spots, and subsequently both the PDMS and parylene layers are peeled off. Using this method, fine patterns of bovine serum albumin (BSA) in arrays of 20 mu m x 20 mu m spots at 2 mu m intervals were achieved. Selective patterning of multiple fluorescent beads was also demonstrated. This method can be performed under the wet condition, which prevents the drying out of proteins and is useful for selective protein patterning with minimal nonspecific binding on the undesired area.