The Tartary Buckwheat Genome Provides Insights into Rutin Biosynthesis and Abiotic Stress Tolerance
The Tartary Buckwheat Genome Provides Insights into Rutin Biosynthesis and Abiotic Stress Tolerance
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苦荞基因组提供了对芦丁生物合成和非生物胁迫耐受性的见解
DOI：
10.1016/j.molp.2017.08.013
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发表时间：
2017-09-12
期刊：
影响因子：
27.5
通讯作者：
Qiao, Zhijun
中科院分区：
文献类型：
--
作者：
Zhang, Lijun;Li, Xiuxiu;Qiao, Zhijun
苦荞麦（Fagopyrum tataricum）是一种重要的假禾谷类作物，具有很强的抗逆性。它的无麸质谷物含有完整的蛋白质和平衡的必需氨基酸组成，是有益的植物化学物质的丰富来源，提供显着的健康益处。在这里，我们报告了一个高质量的，染色体规模的489.3 Mb的苦荞基因组序列，该序列是通过结合Illumina短读段和单分子实时长读段的全基因组鸟枪测序，大型DNA插入fosmid文库的序列标签，Hi-C测序数据和BioNano基因组图谱组装的。我们根据表达证据注释了33366个高置信度的蛋白质编码基因。与甜菜基因组的内部基因组的比较揭示了一个独立的全基因组重复，发生在荞麦血统后，他们从共同的祖先，这是不共享的蔷薇科或甜菜。参考基因组促进了许多新基因的鉴定，这些新基因预计参与芸香苷的生物合成和调控、铝胁迫抗性以及干旱和寒冷胁迫反应。我们的数据表明，苦荞麦的耐受高水平的非生物胁迫的能力是由于参与信号转导，基因调控和膜运输的几个基因家族的扩展。这些基因组资源的获得将有助于发现具有重要农艺和营养价值的基因，并有助于苦荞的遗传改良。
Tartary buckwheat (Fagopyrum tataricum) is an important pseudocereal crop that is strongly adapted to growth in adverse environments. Its gluten-free grain contains complete proteins with a well-balanced composition of essential amino acids and is a rich source of beneficial phytochemicals that provide significant health benefits. Here, we report a high-quality, chromosome-scale Tartary buckwheat genome sequence of 489.3 Mb that is assembled by combining whole-genome shotgun sequencing of both Illumina short reads and single-molecule real-time long reads, sequence tags of a large DNA insert fosmid library, Hi-C sequencing data, and BioNano genome maps. We annotated 33 366 high-confidence protein-coding genes based on expression evidence. Comparisons of the intra-genome with the sugar beet genome revealed an independent whole-genome duplication that occurred in the buckwheat lineage after they diverged from the common ancestor, which was not shared with rosids or asterids. The reference genome facilitated the identification of many new genes predicted to be involved in rutin biosynthesis and regulation, aluminum stress resistance, and in drought and cold stress responses. Our data suggest that Tartary buckwheat's ability to tolerate high levels of abiotic stress is attributed to the expansion of several gene families involved in signal transduction, gene regulation, and membrane transport. The availability of these genomic resources will facilitate the discovery of agronomically and nutritionally important genes and genetic improvement of Tartary buckwheat.