Regeneration of intervertebral discs in a rat disc degeneration model by implanted adipose-tissue-derived stromal cells
Regeneration of intervertebral discs in a rat disc degeneration model by implanted adipose-tissue-derived stromal cells
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DOI：
10.1007/s00701-010-0698-2
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发表时间：
2010-10-01
影响因子：
2.4
通讯作者：
Choi, Kyoung Hyo
中科院分区：
文献类型：
--
作者：
Jeong, Je Hoon;Lee, Jung Hwan;Choi, Kyoung Hyo
由于脂肪组织来源的基质细胞（ADSC）容易获得且干细胞含量丰富，ADSC可能成为细胞治疗和组织工程的更好候选细胞。本研究旨在探讨ADSC移植修复大鼠椎间盘损伤的可能性，将Sprague-Dawley大鼠的第一个尾椎椎间盘节段作为对照组（NC组），其余两个节段用针注射损伤。2周后，将脂肪干细胞（TS）组和生理盐水（IN）组分别移植到两个损伤节段中，6周后TS组椎间盘高度的下降幅度明显小于IN组，MRI信号强度恢复。苏木精和伊红染色显示TS组的内环结构恢复更好。TS组移植后2周抗人核抗体、Ⅱ型胶原和聚集蛋白聚糖染色阳性，ADSCs具有修复退变椎间盘的潜力，可能是治疗IVD的有效方法。
Because adipose-tissue-derived stromal cell (ADSC) is readily accessible and abundant in stem cell, ADSC may be a better candidate for cell therapy and tissue engineering. This study investigated the potential of ADSC implantation to restore disc in a rat IVD model.The first coccygeal disc segments of a Sprague-Dawley rat was left undamaged as a control (NC) group, and other two segments were damaged by needle injection. Two weeks later, ADSCs (TS) group or saline (IN) group was transplanted into each of the two damaged segments.At 6 weeks after transplantation, the TS group showed a significantly smaller reduction in disc height than the IN group and exhibited a restoration of MRI signal intensity. Hematoxylin and eosin staining revealed a greater restoration of the inner annulus structure in the TS group. Anti-Human Nucleic Antibody, collagen type II, and aggrecan, staining showed positive findings at 2 weeks after transplantation in TS group.ADSCs show potential for restoring degenerative discs and may prove effective in the treatment of IVD.