Characterization of extended-spectrum cephalosporins and fluoroquinolone resistance of a Salmonella enterica serovar Thompson isolate from ready-to-eat pork product in China.
Characterization of extended-spectrum cephalosporins and fluoroquinolone resistance of a Salmonella enterica serovar Thompson isolate from ready-to-eat pork product in China.
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中国即食猪肉产品中分离出的肠沙门氏菌 Thompson 血清型的广谱头孢菌素和氟喹诺酮类药物耐药性特征
DOI：
10.3389/fmicb.2022.964009
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发表时间：
2022
影响因子：
5.2
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中科院分区：
文献类型：
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沙门氏菌是全球食源性疾病的主要原因，也是食品安全中的一个共同问题。对超广谱头孢菌素（ESC）和氟喹诺酮（FQs）具有耐药性的肠道沙门氏菌已被世界卫生组织视为高度优先的病原体。对ESC和FQs的共耐药已在S. enterica serovar Thompson（S. Thompson）。然而，在S.汤普森缺乏足够的特征。在这项研究中，我们描述了一个多药耐药（MDR）的S。汤普森分离回收的零售即食（RTE）猪肉产品在中国。基因组的短读段和长读段测序（HiSeq和MinION）确定了blaCMY−2、qnrS 1和qepA 8的存在，沿着11个额外的获得性抗菌素耐药基因，位于152，940 bp的印加/C质粒上。具体来说，blaCMY−2、qnrS 1和qepA 8基因分别位于插入序列（IS）和整合子介导的移动的遗传结构中，sugE-blc-blaCMY−2-ISEc 9、IS 26-orf 6-qnrS 1-orf 5-ISKpn 19和intl 1-qepA 8-orf 10-IS 91-orf 1-dfrA 12-orf 11-aadA 2-qacEΔ1-sul 1。每个基因在不同的细菌物种中被鉴定，表明它们具有高转移能力。该质粒可通过接合转移到大肠杆菌J53中，并导致接合子获得多重抗性。该质粒与来自两个人S.我国不同地区、不同年份分离到的Thompson菌株。此外，还对全球1,868株S. Saintpaul分离物表明，S. Thompson分离株与中国的一株人源分离株在流行病学上高度相关。我们的研究结果表明，中国即食猪肉产品是人类致病性ESCs和FQs共耐S。汤普森此外，这些结果强调了接合质粒在S.汤普森分离物，需要持续调查。
Salmonella is a leading cause of foodborne illness worldwide and is a common concern in food safety. Salmonella enterica displaying resistance to extended-spectrum cephalosporins (ESCs) and fluoroquinolone (FQs) has been deemed a high-priority pathogen by the World Health Organization. Co-resistance to ESCs and FQs has been reported in S. enterica serovar Thompson (S. Thompson). However, the genetic context of ESCs and FQs resistance genes in S. Thompson lacks sufficient characterization. In this study, we characterized a multi-drug resistant (MDR) S. Thompson isolate recovered from a retail ready-to-eat (RTE) pork product in China. Short- and long-read sequencing (HiSeq and MinION) of the genome identified the presence of blaCMY−2, qnrS1, and qepA8, along with 11 additional acquired antimicrobial resistance genes, residing on a 152,940 bp IncA/C plasmid. Specifically, the blaCMY−2, qnrS1, and qepA8 genes were located in insertion sequences (ISs) and integron mediated mobile genetic structure, sugE-blc-blaCMY−2-ISEc9, IS26-orf6-qnrS1-orf5-ISKpn19, and intl1-qepA8-orf10-IS91-orf1-dfrA12-orf11-aadA2-qacEΔ1-sul1, respectively. Each gene was identified in various bacteria species, indicating their high transfer ability. The plasmid was found to be transferable to Escherichia coli J53 by conjugation and resulted in the acquiring of multiple resistances in the transconjugants. The plasmid is closely related to plasmids from two human S. Thompson strains isolated in different regions and years in China. Moreover, core-genome Multi Locus Sequence Typing (cgMLST) and phylogenetic analysis based on global 1,868 S. Saintpaul isolates showed that the S. Thompson isolate was highly epidemiologically linked to a human isolate in China. Our findings suggest that Chinese RTE pork products are a possible source of human pathogenic ESCs and FQs co-resistant S. Thompson. Furthermore, the results underline the important role of conjugative plasmids in acquiring and transmission of ESCs and FQs resistance in S. Thompson isolates, which need continuous investigation.