Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression.
Characterization of porcine ENO3: genomic and cDNA structure, polymorphism and expression.
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猪 ENO3 的表征：基因组和 cDNA 结构、多态性和表达
DOI：
10.1186/1297-9686-40-5-563
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Xiong Y
中科院分区：
文献类型：
--
作者：
Wu J;Zhou D;Deng C;Wu X;Long L;Xiong Y
在本研究中，我们克隆了编码434个氨基酸的猪EN03基因的全长cDNA。它包含12个外显子，大小约为5.4kb。在5‘-非翻译序列中的差异剪接产生两种形式的mRNA，在存在或不存在142个核苷酸片段的情况下，它们彼此不同。表达分析表明，ENO_3的转录本1在肝脏和肺组织中高表达，而转录本2在骨骼肌和心脏中高表达。我们首次提供了约克夏猪和眉山猪在骨骼肌中表达不同的证据。此外，实时定量聚合酶链式反应显示，在约克夏猪中，转录物1在出生后第1天和其他阶段的骨骼肌表达相同，而转录物2的表达水平较高。此外，除肝脏和肾脏外，转录本1在骨骼肌和所有其他组织中的表达均低于转录本2。统计分析表明，中国地方猪品种与引进西方商品猪品种之间存在EN03基因的多态性，并与两个不同群体的脂肪率、平均背脂厚度、大理石花纹和肌内脂肪有关。
In this study, a full-length cDNA of the porcine ENO3 gene encoding a 434 amino acid protein was isolated. It contains 12 exons over approximately 5.4 kb. Differential splicing in the 5'-untranslated sequence generates two forms of mRNA that differ from each other in the presence or absence of a 142-nucleotide fragment. Expression analysis showed that transcript 1 of ENO3 is highly expressed in liver and lung, while transcript 2 is highly expressed in skeletal muscle and heart. We provide the first evidence that in skeletal muscle expression of ENO3 is different between Yorkshire and Meishan pig breeds. Furthermore, real-time polymerase chain reaction revealed that, in Yorkshire pigs, skeletal muscle expression of transcript 1 is identical at postnatal day-1 and at other stages while that of transcript 2 is higher. Moreover, expression of transcript 1 is lower in skeletal muscle and all other tissue samples than that of transcript 2, with the exception of liver and kidney. Statistical analysis showed the existence of a polymorphism in the ENO3 gene between Chinese indigenous and introduced commercial western pig breeds and that it is associated with fat percentage, average backfat thickness, meat marbling and intramuscular fat in two different populations.