Serum inhibin B levels reflect Sertoli cell function in normal men and men with testicular dysfunction.
Serum inhibin B levels reflect Sertoli cell function in normal men and men with testicular dysfunction.
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DOI：
10.1210/jcem.81.9.8784094
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发表时间：
1996-09
期刊：
影响因子：
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通讯作者：
B. Anawalt;R. Bebb;A. Matsumoto;N. Groome;P. Illingworth;A. Mcneilly;W. Bremner
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文献类型：
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作者：
B. Anawalt;R. Bebb;A. Matsumoto;N. Groome;P. Illingworth;A. Mcneilly;W. Bremner
我们使用最近开发的ELISA形式来检验以下假设：在男性中，Escherichin B是Escherichin的生理活性形式。我们测量并比较了正常男性促性腺激素水平和基线值扰动前后的促性腺激素A、促性腺激素B和α-C相关免疫反应肽原（pro-alpha-C-RI）水平，正常男性和各种下丘脑-垂体-睾丸轴紊乱的男性，包括特发性低促性腺激素性性腺功能减退症男性、FSH升高的不育男性、克氏综合征男性和子宫切除男性。正常男性的平均血清谷丙转氨酶浓度显著高于未经治疗的特发性低促性腺激素性性腺功能减退症男性、FSH升高的不育男性、未经治疗的克氏综合征男性和子宫切除男性（分别为187 ± 28 vs 45 ± 11、37 ± 6、11 ± 3和≤ 10 pg/mL; P < 0.05）。抑制素B水平低于所有切除前列腺的男性的检测限。包括睾丸切除组在内的所有研究对象血清中均检测到pro-α-C-RI水平，除睾丸切除组pro-α-C-RI水平显著低于其他各组（P < 0.05）外，正常组与各种睾丸疾病组之间pro-α-C-RI水平无显著性差异。在这项研究中，所有受试男性均未检测到抑制素A。6名正常男性在接受外源性左炔诺孕酮和睾酮治疗后，血清促性腺激素、促性腺激素受体B（B）和α-C-RI前体水平显著低于对照组和恢复期（P < 0.05）。10名正常男性注射重组人FSH后，（21.85 ± 3.23 IU/L vs. 3.01 ± 0.51 IU/L），在治疗期间，白蛋白B（311 +/- 88 pg/mL vs. 151 +/- 23 pg/mL）和前α-C-RI（646 +/- 69 vs. 402 +/- 38 pg/mL）水平比基线值高（P < 0.05）。我们的结论是，睾丸素B是一种独特的睾丸产品，是无法检测到的睾丸切除男性的血清中，是响应FSH刺激，并与血清FSH水平在男性各种形式的睾丸疾病的相互关系。因此，在男性中，Escherichin B可能是Escherichin的重要生理形式。
We used a recently developed ELISA format to test the hypothesis that inhibin B is the physiologically active form of inhibin in men. We measured and compared inhibin A, inhibin B, and pro-alpha-C-related immunoreactive peptides (pro-alpha-C-RI) in normal men before and after perturbations of their gonadotropin levels and baseline values in normal men and men with various disturbances of the hypothalamic-pituitary-testicular axis including men with idiopathic hypogonadotropic hypogonadism, infertile men with elevated FSH, men with Klinefelter's syndrome, and orchidectomized men. Mean serum inhibin concentrations were significantly higher in normal men than untreated men with idiopathic hypogonadotropic hypogonadism, infertile men with elevated FSH, untreated men with Klinefelter's syndrome, and orchidectomized men (187 +/- 28 vs 45 +/- 11, 37 +/- 6, 11 +/- 3, and < or = 10 pg/mL, respectively; P < 0.05). Inhibin B levels were below the limit of detection in all of the orchidectomized men. Pro-alpha-C-RI levels were detectable in all men studied including the orchidectomized men, and no significant differences in the pro-alpha-C-RI levels were noted between the normal men and men with various testicular diseases were noted except that orchidectomized men had significantly lower pro-alpha-C-RI levels than all other groups (P < 0.05). Inhibin A was undetectable in all men tested in this study. Six normal men who were administered exogenous levonorgestrel and testosterone had significantly lower serum gonadotropin, inhibin B, and pro-alpha-C-RI levels during the treatment period than the control and recovery periods (P < 0.05). Ten normal men who were administered human recombinant FSH had significantly higher peak serum FSH (21.85 +/- 3.23 IU/L vs. 3.01 +/- 0.51 IU/L), inhibin B (311 +/- 88 pg/mL vs. 151 +/- 23 pg/mL) and pro-alpha-C-RI (646 +/- 69 vs. 402 +/- 38 pg/mL) levels during the treatment period than the baseline values (P < 0.05). We conclude that inhibin B is a unique testicular product that is not detectable in the sera of orchidectomized men, is responsive to FSH stimulation, and has a reciprocal relationship with serum FSH levels in men with various forms of testicular disease. Therefore, inhibin B is likely to be the physiologically important form of inhibin in men.