Multilineage potential of adult human mesenchymal stem cells
Multilineage potential of adult human mesenchymal stem cells
复制标题
DOI：
10.1126/science.284.5411.143
复制
发表时间：
1999-04-02
期刊：
影响因子：
56.9
通讯作者：
Marshak, DR
中科院分区：
文献类型：
--
作者：
Pittenger, MF;Mackay, AM;Marshak, DR
人间充质干细胞被认为是多能细胞，其存在于成人骨髓中，可以作为未分化细胞复制，并且具有分化成间充质组织谱系的潜力，包括骨、软骨、脂肪、肌腱、肌肉和骨髓基质。具有人间充质干细胞特征的细胞从志愿者供体的骨髓抽吸物中分离。这些细胞表现出稳定的表型，并在体外保持为单层。这些成体干细胞可以被诱导分化成脂肪细胞、软骨细胞或骨细胞谱系。个体干细胞被鉴定为当扩增成集落时，保留其多谱系潜能。
Human mesenchymal stem cells are thought to be multipotent cells, which are present in adult marrow, that can replicate as undifferentiated cells and that have the potential to differentiate to Lineages of mesenchymal tissues, including bone; cartilage, fat, tendon, muscle, and marrow stroma. Cells that have the characteristics of human mesenchymal stem cells were isolated from marrow aspirates of volunteer donors. These cells displayed a stable phenotype and remained as a monolayer in vitro. These adult stem cells could be induced to differentiate exclusively into the adipocytic, chondrocytic, or osteocytic Lineages. Individual stem cells were identified that, when expanded to colonies, retained their multilineage potential.