Identification of minimum Rpn4‐responsive elements in genes related to proteasome functions
Identification of minimum Rpn4‐responsive elements in genes related to proteasome functions
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DOI：
10.1016/j.febslet.2015.02.025
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发表时间：
2015-04
期刊：
影响因子：
3.5
通讯作者：
Ryohei Shirozu;H. Yashiroda;S. Murata
中科院分区：
文献类型：
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作者：
Ryohei Shirozu;H. Yashiroda;S. Murata
蛋白酶体是介导泛素依赖性蛋白水解的必需的66亚基蛋白酶。转录因子Rpn 4通过识别启动子区域上的九聚体蛋白酶体相关控制元件（PACE）元件来调节蛋白酶体亚基的协同表达以增加蛋白酶体。然而，蛋白酶体组装分子伴侣和一些亚基的基因没有PACEs。在这里，我们确定了一个最小的六聚体“PACE-core”序列，响应Rpn 4。PACE-core存在于许多与蛋白酶体功能相关的基因中，包括组装分子伴侣，但不能取代PACE亚基。我们的研究结果增加了一个新的复杂性，在转录调控的基因参与蛋白质降解。
The proteasome is an essential, 66-subunit protease that mediates ubiquitin-dependent proteolysis. The transcription factor Rpn4 regulates concerted expression of proteasome subunits to increase the proteasome by recognizing nonamer proteasome-associated control element (PACE) elements on the promoter regions. However, the genes for proteasome assembly chaperones and some of the subunits have no PACEs. Here we identified a minimal hexamer “PACE-core” sequence that responds to Rpn4. PACE-cores are found in many genes related to proteasome function including the assembly chaperones, but cannot substitute for PACE of the subunits. Our results add a new layer of complexity in transcriptional regulation of genes involved in protein degradation.