Na+ channel regulation by calmodulin kinase II in rat cerebellar granule cells
Na+ channel regulation by calmodulin kinase II in rat cerebellar granule cells
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DOI：
10.1006/bbrc.2000.3145
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发表时间：
2000-08-02
影响因子：
3.1
通讯作者：
Couraud, F
中科院分区：
文献类型：
--
作者：
Carlier, E;Dargent, B;Couraud, F
研究了特异性钙调蛋白激酶Ⅱ抑制剂对大鼠小脑颗粒细胞Na+通道的影响。KN-62的最大效果在20 μ M时观察到，仅在10分钟应用后，峰值Na+电流就减少了80%。在稳态失活中也观察到8 mV的超极化位移。KN-04（20 μ M），一种无活性的类似物，没有检测到的影响。然而，KN-62对表达II A型α亚基的中国仓鼠卵巢细胞记录的Na+电流无活性。我们还分析了CaM激酶II 296-311和CaM激酶II 281-309肽的抑制作用。这两种肽（75 μ M）诱导的最大峰值Na+电流减少在30分钟内。在类似的条件下，截短的肽钙调素激酶II 284-302是无效的。这些结果表明，钙调素I激酶II作为一个调制器的Na+通道活性在小脑颗粒细胞。(C)北京大学出版社.
The effects of specific CaM kinase II inhibitors were investigated on Na+ channels from rat cerebellar granule tells. A maximal effect of KN-62 was observed at 20 mu M and consisted of an 80% reduction of the peak Na+ current after only a 10-min application. A hyperpolarizing shift of 8 mV in the steady-state inactivation was also observed. KN-04 (20 mu M), an inactive analog, had no detectable effect. KN-62 was however inactive on Na+ currents recorded from Chinese hamster ovary cells expressing the type II A alpha subunit. We have also analyzed the inhibitory effects of CaM kinase II 296-311 and CaM kinase II 281-309 peptides. Both peptides (75 mu M) induced a maximum peak Na+ current reduction within 30 min. Under similar conditions, a truncated peptide CaM kinase II 284-302 was ineffective. These results demonstrate that CaM;I kinase II acts as a modulator of Na+ channel activity in cerebellar granule cells. (C) 2000 Academic Press.