Detection of cell detachment activity induced by Moraxella bovis.
Detection of cell detachment activity induced by Moraxella bovis.
复制标题
牛莫拉氏菌诱导的细胞脱离活性的检测。
DOI：
10.2460/ajvr.2000.61.1145
复制
发表时间：
2000
影响因子：
1
通讯作者：
L. Riley
中科院分区：
文献类型：
--
作者：
R. Marrion;L. Riley
目的
表征从牛莫拉氏菌培养物获得的滤液对培养的角膜上皮细胞和其他类型的培养的哺乳动物细胞的影响。
样本群体
培养的仓鼠角膜上皮细胞、牛上皮细胞和几种转化细胞系暴露于牛支原体致病性分离株的培养滤液。
程序
培养牛莫拉氏菌，过滤除菌。将所得细菌培养物滤液与各种类型的培养细胞一起孵育，并通过使用中性红染料定量滤液对各种哺乳动物细胞类型的脱离的影响。此外，在与培养的哺乳动物细胞一起孵育之前，用蛋白酶抑制剂以及胰蛋白酶处理细菌培养物滤液并加热。
结果
牛支原体的细菌培养物滤液导致所有类型的培养细胞彼此分离并与基质分离，在开始孵育后2小时最大效果明显。分离的细胞是活的，并且分离是可逆的。丝氨酸蛋白酶抑制剂（苯甲基磺酰氟和α 2-巨球蛋白）抑制细胞脱落归因于细菌培养滤液。加热和用胰蛋白酶处理也抑制细胞脱离。
结论和临床相关性
牛莫拉氏菌产生一种可溶性因子，导致培养细胞可逆性脱落。这种活性可能在传染性牛角膜结膜炎的发病机制中起作用。
OBJECTIVE
To characterize the effect that filtrate obtained from cultures of Moraxella bovis has on cultured corneal epithelial cells and other types of cultured mammalian cells.
SAMPLE POPULATION
Cultured hamster corneal epithelial cells, bovine epithelial cells, and several transformed cell lines exposed to culture filtrate from a pathogenic isolate of M bovis.
PROCEDURE
Moraxella bovis was cultured, and bacteria were removed by filtration. The resulting bacterial culture filtrate was incubated with various types of cultured cells, and effects of the filtrate on detachment of various mammalian cell types was quantified by the use of neutral red dye. Additionally, bacterial culture filtrate was treated with protease inhibitors as well as trypsin and heat prior to incubation with cultured mammalian cells.
RESULTS
Bacterial culture filtrate of M bovis caused all types of cultured cells to detach from each other and from the substrate, with the maximal effect evident 2 hours after initiating incubation. Detached cells were alive, and detachment was reversible. Serine protease inhibitors (phenylmethylsulfonylfluoride and alpha2-macroglobulin) inhibited cell detachment attributable to bacterial culture filtrate. Heating and treatment with trypsin also inhibited cell detachment.
CONCLUSIONS AND CLINICAL RELEVANCE
Moraxella bovis produces a soluble factor that causes reversible detachment of cultured cells. This activity may play a role in the pathogenesis of infectious bovine keratoconjunctivitis.
影响因子：
4.4
作者：
Gipson,IK;Grill,SM
通讯作者：
Grill,SM