Drosophila genes encoding maternal-specific and maternal-differential RNAs.
Drosophila genes encoding maternal-specific and maternal-differential RNAs.
复制标题
果蝇基因编码母体特异性和母体差异RNA。
DOI：
10.1002/dvg.1020090104
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Lengyel,JA
中科院分区：
文献类型：
--
作者：
Underwood,EM;Lengyel,JA
在果蝇胚胎发生的最初几个小时内发生的独特的细胞和遗传事件表明，有些基因的功能完全或大部分局限于这一阶段;遗传和分子证据都支持这一点。为了鉴定这些基因中的一些并表征特异性母本和特异性合子转录对早期胚胎发生的相对贡献，我们使用果蝇基因组DNA文库的竞争和差异筛选来获得胚盘和母本差异序列[Roark et al.：Dev Biol 109：476-488，1985]。我们在这里描述的EcoRI限制性片段，染色体的位置，和大小和发育模式的表达的RNA转录从19母差序列。五个序列编码母体特异性转录物（在母体RNA中比在任何其他阶段丰富50-150倍）。母体特异性和母体差异性序列位于所有主要染色体臂上的单个位点。这些网站与目前映射的母效应基因的网站的比较显示了几种可能的相关性，包括一个区域包含三个母效应致死突变和两个maternalspecific序列。
The unique cellular and genetic events which occur during the first few hours of Drosophila embryogenesis suggest that there are genes whose function is entirely or largely limited to this stage; this is supported by both genetic and molecular evidence. To identify some of these genes and characterize the relative contribution of specifically maternal and specifically zygotic transcription to early embryogenesis, we used competition and differential screening of a Drosophila genomic DNA library to obtain blastoderm-and maternal-differential sequences [Roark et al.: Dev Biol 109: 476–488, 1985]. We describe here the Eco RI restriction fragments, chromosomal location, and size and developmental pattern of expression of the RNAs transcribed from 19 maternal-differential sequences. Five sequences encode maternal-specific transcripts (50-150-fold more abundant in maternal RNA than at any other stage). The maternal-specific and maternal-differential sequences are located at single sites on all major chromosome arms. Comparison of these sites with the sites of presently mapped maternal-effect genes shows several possible correlations, including one region containing three maternal-effect lethal mutations and two maternalspecific sequences.