Evaluation of vascular endothelial growth factor levels in tears and serum among diabetic patients
Evaluation of vascular endothelial growth factor levels in tears and serum among diabetic patients
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DOI：
10.1371/journal.pone.0221481
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发表时间：
2019-08-22
期刊：
影响因子：
3.7
通讯作者：
Mahaneem, Mohamed
中科院分区：
文献类型：
--
作者：
Ang, Wen Jeat;Zunaina, Embong;Mahaneem, Mohamed
检测眼组织中血管内皮生长因子（VEGF）水平可能有助于了解VEGF在糖尿病视网膜病变（DR）发病机制和进展中的作用。本研究的目的是评估2型糖尿病（DM）患者泪液和血清中VEGF的水平。方法2016年8月至2018年5月进行了一项比较性横断面研究，涉及无DR、非增殖性DR（NPDR）和增殖性DR（PDR）的2型DM患者。使用41号Whatman滤纸（Schirmer条）收集泪液样品，并通过静脉穿刺抽取5 mL血液样品。采用酶联免疫吸附试验（ELISA）检测泪液和血清中VEGF水平。结果共88例2型糖尿病患者（无DR 30例，NPDR 28例，PDR 30例）入选本研究。NPDR和PDR组的平均泪液VEGF水平（分别为114.4 SD 52.5 pg/mL和150.8 SD 49.7 pg/mL）显著高于无DR组（40.4 SD 26.5 pg/mL，p
ObjectiveDetection of vascular endothelial growth factor (VEGF) levels in ocular tissue may perhaps provide insight into the role of VEGF in the pathogenesis and progression of diabetic retinopathy (DR). The aim of this study was to evaluate the levels of VEGF in tears and serum amongst type 2 diabetes mellitus (DM) patients.MethodsA comparative cross-sectional study was conducted between August 2016 and May 2018 involving type 2 DM patients with no DR, non-proliferative DR (NPDR), and proliferative DR (PDR). Tear samples were collected using no.41 Whatman filter paper (Schirmer strips) and 5 mL blood samples were drawn by venous puncture. VEGF levels in tears and serum were measured by enzyme-linked immunosorbent assay.ResultsA total of 88 type 2 DM patients (no DR: 30 patients, NPDR: 28 patients, PDR: 30 patients) were included in the study. Mean tear VEGF levels were significantly higher in the NPDR and PDR groups (114.4 SD 52.5 pg/mL and 150.8 SD 49.7 pg/mL, respectively) compared to the no DR group (40.4 SD 26.5 pg/mL, p