Mesenchymal Stem Cells Derived from Human Adipose Tissue
Mesenchymal Stem Cells Derived from Human Adipose Tissue
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DOI：
10.1007/978-1-4939-2938-2_4
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发表时间：
2015-01-01
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影响因子：
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通讯作者：
Doran, Pauline M.
中科院分区：
文献类型：
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作者：
Mahmoudifar, Nastaran;Doran, Pauline M.
人类成体间充质干细胞存在于脂肪组织中，可以使用外科手术如吸脂术获得。间充质干细胞的多谱系能力使其对于基于细胞的医学疗法非常有价值。在这一章中，我们描述了如何从成人脂肪组织中分离间充质干细胞，在培养中增殖细胞，并冷冻保存细胞用于组织工程应用。还描述了用于脂肪来源的干细胞的鉴定和表征以及用于细胞分选的流式细胞术方法。
Human adult mesenchymal stem cells are present in fat tissue, which can be obtained using surgical procedures such as liposuction. The multilineage capacity of mesenchymal stem cells makes them very valuable for cell-based medical therapies. In this chapter, we describe how to isolate mesenchymal stem cells from human adult fat tissue, propagate the cells in culture, and cryopreserve the cells for tissue engineering applications. Flow cytometry methods are also described for identification and characterization of adipose-derived stem cells and for cell sorting.