Evaluating potential therapies for bladder dysfunction in a mouse model of multiple sclerosis with high-resolution ultrasonography
Evaluating potential therapies for bladder dysfunction in a mouse model of multiple sclerosis with high-resolution ultrasonography
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DOI：
10.1177/1352458509104594
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发表时间：
2009-07-01
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影响因子：
--
通讯作者：
Baker, D.
中科院分区：
文献类型：
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作者：
Al-Izki, S.;Pryce, G.;Baker, D.
在多发性硬化症（MS）中，脱髓鞘和神经退行性病变在整个中枢神经系统中发展，导致神经传递丧失。因此，多发性硬化症患者表现出多种症状，包括膀胱功能障碍，这可能导致尿失禁或尿潴留。这些症状可以在多发性硬化症的动物模型中出现。目前对动物模型膀胱特性的评估通常是侵入性的电生理技术。目的采用无创超声测量ms模型小鼠膀胱容量方法诱导小鼠慢性复发性实验性自身免疫性脑脊髓炎。在疾病过程中以及用氯化亚酚治疗后，用超声检查膀胱体积。结果超声测得的尿量与手工测得的尿量呈正相关（r = 0.960）。研究还首次表明，残留神经缺陷的小鼠膀胱大小显著增加。事实上，膀胱大小的增加与后肢痉挛表现出很强的正相关（r = 0.951）。慢性实验性自身免疫性脑脊髓炎小鼠膀胱体积显著减少。结论：本研究展示了一种新的实验性质谱结果测量方法，它允许；重复、无创、高分辨率膀胱功能超声监测。多发性硬化症2009；15: 795 - 801。http://msj.sagepub.com
Background In multiple sclerosis (MS), demyelinating and neurodegenerative lesions develop throughout the central nervous system, which result in a loss of neurotransmission. As a result, people with MS exhibit a wide range of symptoms including dysfunction of the bladder, which can lead to urinary incontinence or retention. Such signs can develop in animal models of MS. Current assessments of bladder properties in animal models are generally invasive, electrophysiological techniques.Objective The use of a non-invasive, ultrasound system for measuring bladder volume in a mouse model of MS.Methods Chronic relapsing experimental autoimmune encephalomyelitis was induced in mice. The bladder volume was assessed using ultrasonography, during the disease course and following therapy with bethanechol chloride.Results It was demonstrated that volumes obtained ultrasonically positively-correlated (r = 0.960) with the urine volumes obtained by manual expression. It was also shown for the first time that bladder size increased significantly in mice with residual neurological deficit. Indeed, this increase in bladder size showed a strong, positive-correlation (r = 0.951) with the hind limb spasticity. Following treatment with bethanechol chloride, bladder volume significantly decreased in mice with chronic experimental autoimmune encephalomyelitis.Conclusion This study demonstrates a novel outcome measure in experimental MS that allows; repeated, non-invasive, high resolution ultrasonic monitoring of bladder function. Multiple Sclerosis 2009; 15: 795-801. http://msj.sagepub.com