Chemical shift assignments of CHU_1110: an AHSA1-like protein from Cytophaga hutchinsonii

Chemical shift assignments of CHU_1110: an AHSA1-like protein from Cytophaga hutchinsonii
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CHU_1110 的化学位移分配:来自 Cytophaga hutchinsonii 的 AHSA1 样蛋白

DOI:
10.1007/s12104-018-9799-2
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发表时间:
2018-01
影响因子:
0.9
通讯作者:
Liu Maili
Liu Maili
中科院分区:
生物学4区
文献类型:
--
作者:
Liang Chunjie;He Ting;Li Tao;Yang Yunhuang;Zhu Jiang;Liu Maili

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AHSA1 蛋白家族是 Bet v1 样蛋白超家族中四个最大的家族之一。 AHSA1 家族中蛋白质的功能和结构仍然很大程度上未知。 CHU_1110 含有 167 个氨基酸,分子量为 19.2 kDa,是来自哈钦森细胞噬菌体 (Cytophaga hutchinsonii) 的 AHSA1 家族成员,这是一种以消化结晶纤维素能力而闻名的土壤细菌。在这里,我们报告了 CHU_1110 的完整 1H、13C 和 15N 化学位移分配。 CGL2373的二级结构元件与规范的AHSA1结构一致。然而,CHU_1110 与具有功能和结构报告的 AHSA1 家族其他成员(例如来自根瘤菌的 RHE_CH02687、来自智人的 Aha1 和来自枯草芽孢杆菌的 Yndb)的序列同一性非常低,这可能表明 CHU_1110 具有不同的功能。我们对 CHU_1110 的化学位移分配对于 CHU_1110 的后续结构和功能研究至关重要。
AHSA1 protein family is one of the four largest families in the Bet v1-like protein superfamily. The functions and structures of proteins in AHSA1 family are still largely unknown. CHU_1110 with 167 amino acids and a molecular weight of 19.2 kDa is a member of the AHSA1 family from Cytophaga hutchinsonii, a soil bacterium known for its ability to digest crystalline cellulose. Here we report the complete1H,13C and15N chemical shift assignments of CHU_1110. The secondary structural elements of CGL2373 are consistent with the canonical AHSA1 structure. However the sequence identity of CHU_1110 with other members of AHSA1 family with functional and structural reports, such as RHE_CH02687 from Rhizobium etli, Aha1 from Homo sapiens and Yndb from Bacillus subtilis, are very low, which may suggest a different function of CHU_1110. Our chemical shift assignments of CHU_1110 are essential for the following structural and functional research of CHU_1110.
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