Design, development, and performance comparison of wide field lensless and lens-based optical systems for point-of-care biological applications
Design, development, and performance comparison of wide field lensless and lens-based optical systems for point-of-care biological applications
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DOI：
10.1016/j.optlaseng.2020.106326
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发表时间：
2021-02-01
影响因子：
4.6
通讯作者：
Asghar, Waseem
中科院分区：
文献类型：
--
作者：
Fennell, Robert D.;Sher, Mazhar;Asghar, Waseem
无透镜生物成像系统是传统显微系统的一种新兴替代方案，因为它们能够实现宽视场成像。大多数显微系统为了放大倍数而牺牲了视场，而无透镜系统利用了小成像像素尺寸、投影、数字放大和后处理来补偿衍射图像。一种新的基于透镜的系统被设计为具有与基本无透镜装置完全相同的宽视场。一种新的复合透镜系统设计被用于实现一个明确的目标，即拥有与无透镜装置相同的视场。然后，在这种复杂程度下比较这两种光学成像装置（无透镜和基于透镜的装置）的特性，以了解为了实现生物成像目标，对于简化且成本更低的未来设计需要哪些最小系统原理。对于这两种成像系统，生物实体的图像都是在相同的CMOS成像设备和计算机软件的帮助下记录的。这项工作的主要贡献是使用光学标准和生物图像对两种系统的性能特征进行了详尽的比较。
Lensless biological imaging systems are an emerging alternative to conventional microscopic systems because they enable a wide field of view imaging. While most microscopic systems sacrifice the field of view for magnification, lensless systems have taken advantage of small imaging pixel size, projection, digital magnification, and post-processing to compensate for diffracted images. A new lens-based system is designed to have the exact same wide field of view as that of a basic lensless setup. A new compound lens system design is utilized to achieve an explicit aim to have the same fields of view as the lensless setup. Then the characteristics of these two optical imaging setups (lensless and lens-based setups) are compared at this level of complexity to see what the minimal systems principles are needed to achieve the biological imaging goals for simplified and less expensive future designs. For both imaging systems, images of biological entities are recorded with the help of the same CMOS imaging device and computer software. The main contribution of this work is an exhaustive comparison between the performance characteristics of both systems using optical standards and biological images.