Characterization of Kupffer cells in livers of developing mice.
Characterization of Kupffer cells in livers of developing mice.
复制标题
DOI：
10.1186/1476-5926-10-2
复制
发表时间：
2011-07-12
期刊：
影响因子：
--
通讯作者：
Robertson RT
中科院分区：
文献类型：
--
作者：
Lopez BG;Tsai MS;Baratta JL;Longmuir KJ;Robertson RT
库普弗细胞是众所周知的肝脏巨噬细胞，然而，库普弗细胞在小鼠中的发育特征还不清楚。为了阐明这一问题，库普弗巨噬细胞在正常发育的小鼠肝脏的特点进行了研究，使用光学显微镜和免疫细胞化学。采用免疫细胞化学技术制备了出生后早期小鼠的肝组织切片。通过枯否细胞对F4/80抗体的免疫反应性鉴定枯否细胞，而内皮细胞用CD-34抗体标记。此外，枯否细胞和内皮细胞标记的全身注射荧光标记乳胶微球。通过荧光显微镜检查组织切片。静脉或腹膜内注射微球产生类似的肝细胞标记模式。F4/80阳性Kupffer细胞用大（0.2 μm）和小（0.02 μm）直径的微球标记，而内皮细胞仅用较小直径的微球标记。枯否细胞的微球标记似乎稳定至少6周。早在出生后第0天就鉴定出F4/80免疫反应性细胞，这些细胞也显示出微球摄取。F4/80枯否细胞数量（相对于白蛋白阳性肝细胞数量）在出生后前2周内未显示出显著趋势。发育中的小鼠肝脏的枯否细胞与其他哺乳动物的枯否细胞非常相似，这证实了小鼠为研究肝脏巨噬细胞发育结构和功能提供了有用的动物模型。
Kupffer cells are well known macrophages of the liver, however, the developmental characteristics of Kupffer cells in mice are not well understood. To clarify this matter, the characteristics of Kupffer macrophages in normal developing mouse liver were studied using light microscopy and immunocytochemistry. Sections of liver tissue from early postnatal mice were prepared using immunocytochemical techniques. The Kupffer cells were identified by their immunoreactivity to the F4/80 antibody, whereas endothelial cells were labelled with the CD-34 antibody. In addition, Kupffer cells and endothelial cells were labelled by systemically injected fluorescently labelled latex microspheres. Tissue slices were examined by fluorescence microscopy. Intravenous or intraperitonal injections of microspheres yielded similar patterns of liver cell labelling. The F4/80 positive Kupffer cells were labelled with both large (0.2 μm) and small (0.02 μm) diameter microspheres, while endothelial cells were labelled only with the smaller diameter microspheres. Microsphere labelling of Kupffer cells appeared stable for at least 6 weeks. Cells immunoreactive for F4/80 were identified as early as postnatal day 0, and these cells also displayed uptake of microspheres. Numbers of F4/80 Kupffer cells, relative to numbers of albumin positive hepatocytes, did not show a significant trend over the first 2 postnatal weeks. Kupffer cells of the developing mouse liver appear quite similar to those of other mammalian species, confirming that the mouse presents a useful animal model for studies of liver macrophage developmental structure and function.