Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice
Estrogen configures sexual dimorphism in the preoptic area of C57BL/6J and ddN strains of mice
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DOI：
10.1002/cne.22419
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发表时间：
2010-09
影响因子：
2.5
通讯作者：
C. Orikasa;Y. Sakuma
中科院分区：
文献类型：
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作者：
C. Orikasa;Y. Sakuma
使用钙结合蛋白D28 k抗体的免疫组织化学显示，在C57 BL/6 J和ddN小鼠品系的内侧视前区的中央部分的神经元分布的显着性别差异时，动物被处死的D 65（D1 =出生当天）。雄性小鼠具有明显的椭圆形细胞聚集体，而雌性小鼠缺乏这种结构。在尼氏染色切片中未观察到这种性别差异。钙结合蛋白D28 k和神经元特异性核蛋白NeuN的共定位证实聚集体中的细胞是神经元。在两种菌株中，雄性的聚集体大于雌性。当在D 65观察时，在D1切除睾丸的雄性动物具有较小的聚集体。然而，在D1-D5期间每日注射2 μg苯甲酸雌二醇以及在D1时单次注射100 μg丙酸睾酮使雌性聚集体增大，但在D1时单次注射100 μg双氢睾酮对雌性表型没有影响。类似的内分泌操作对两种性别的成年动物均无影响。因此，C57 BL/6 J和ddN小鼠视前区的钙结合蛋白免疫反应细胞聚集体在形态学和性类固醇依赖性组织方面与大鼠视前区的性二态核同源。J. Comp.神经元518：3618-3629，2010.© 2010 Wiley利斯公司
Immunohistochemistry using a calbindin D28k antibody revealed a marked sex difference in neuronal distribution in the central portion of the medial preoptic area in C57BL/6J and ddN strains of mice when the animals were sacrificed on D65 (D1 = the day of birth). Male mice had a distinct ellipsoidal cell aggregate, whereas females lacked such a structure. This sex difference was not observed in Nissl‐stained sections. Co‐localization of calbindin D28k and the neuron‐specific nuclear protein NeuN confrmed that the cells in the aggregate were neurons. The aggregates were larger in males than in females in both strains. When observed on D65, males orchidectomized on D1 had smaller aggregates. However, daily injections of 2 μg estradiol benzoate through D1–D5 as well as a single injection of 100 μg testosterone propionate on D1 enlarged the aggregates in females, but a single injection of 100 μg dihydrotestosterone on D1 had no effect on the female phenotype. Similar endocrine manipulations had no effects in adult animals of both sexes. Thus, the calbindin‐immunoreactive cell aggregates in the preoptic area of C57BL/6J and ddN mice are homologous to the sexually dimorphic nucleus of the rat preoptic area in terms of the morphology and sex steroid‐dependent organization. J. Comp. Neurol. 518:3618–3629, 2010. © 2010 Wiley‐Liss, Inc.