Molecular analysis of 11q13 breakpoints in multiple myeloma
Molecular analysis of 11q13 breakpoints in multiple myeloma
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DOI：
10.1182/blood.v93.4.1330.404k04_1330_1337
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发表时间：
1999-02-15
期刊：
影响因子：
20.3
通讯作者：
Neri, A
中科院分区：
文献类型：
--
作者：
Ronchetti, D;Finelli, P;Neri, A
t（11; 14）（q13; q32）染色体易位是套细胞淋巴瘤（MCL）的标志，在大约30%的多发性骨髓瘤（MM）肿瘤中发现14 q32易位。尽管已经发现细胞周期蛋白D1的过表达与携带t（11; 14），经常参与MCL的BCL-1/细胞周期蛋白D1区域的重排很少发生在MM细胞系或原发性肿瘤中。为了测试MM中是否可能出现特定的11 q13断点簇，我们使用源自MM相关11 q13断点的探针通过Southern印迹分析研究了一组代表性的原发性肿瘤。为此，我们首先克隆了来自原发性肿瘤和U266细胞系的断裂点和相应的生殖系区域，以及来自KMS-12细胞系的生殖系区域，使用大组探针测试了来自50个原发性肿瘤的DNA，但是使用KMS-12断裂点探针仅在一个病例中检测到重排。我们的研究结果证实了先前的发现，即MM中的11 q13断裂点分散在包括细胞周期蛋白D1基因的11 q13区域中，从而表明MM中不存在11 q13断裂点簇。
The t(11;14)(q13;q32) chromosomal translocation, which is the hallmark of mantle cell lymphoma (MCL), is found in approximately 30% of multiple myeloma (MM) tumors with a 14q32 translocation, Although the overexpression of cyclin D1 has been found to be correlated with MM cell lines carrying the t(11;14), rearrangements of the BCL-1/cyclin D1 regions frequently involved in MCL rarely occur in MM cell lines or primary tumors. To test whether specific 11q13 breakpoint clusters may occur in MM, we investigated a representative panel of primary tumors by means of Southern blot analysis using probes derived from MM-associated 11q13 breakpoints. To this end, we first cloned the breakpoints and respective germ-line regions from a primary tumor and the U266 cell line, as well as the germ-line region from the KMS-12 cell line, DNA from 50 primary tumors was tested using a large panel of probes, bur a rearrangement was detected in only one case using the KMS-12 breakpoint probe. Our results confirm previous findings that the 11q13 breakpoints in MM are scattered throughout the 11q13 region encompassing the cyclin D1 gene, thus suggesting the absence of 11q13 breakpoint clusters in MM. (C) 1999 by The American Society of Hematology.