Generalizing, Extending, and Maximizing Nitrogen-15 Hyperpolarization Induced by Parahydrogen in Reversible Exchange
Generalizing, Extending, and Maximizing Nitrogen-15 Hyperpolarization Induced by Parahydrogen in Reversible Exchange
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DOI：
10.1021/acs.jpcc.6b12097
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发表时间：
2017-03-30
影响因子：
3.7
通讯作者：
Theis, Thomas
中科院分区：
文献类型：
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作者：
Colell, Johannes F. P.;Logan, Angus W. J.;Theis, Thomas
可逆交换信号放大（SABRE）是一种快速、方便的核磁共振超极化方法，它使用廉价、易得的对氢作为超极化源。SABRE能使质子和异核超极化。在这里，我们将重点介绍作为SABRE- sheath的异核变异（SABRE在SHield中允许向异核转移），特别是氮-15靶标。我们发现N-15-SABRE比H-1-SABRE更有效，在更广泛的衬底上工作，极大地推广了SABRE方法。此外，我们发现氮-1可以显著延长T-1倍，最长可达12分钟。长T-1次可以实现更高的超极化水平，但也有望实现几十分钟的超极化分子成像。详细的表征和优化提出，导致氮-15极化率超过10%的几个化合物。
Signal Amplification by Reversible Exchange (SABRE) is a fast and convenient NMR hyperpolarization method that uses cheap and readily available para-hydrogen as a hyperpolarization source. SABRE can hyperpolarize protons and heteronuclei. Here we focus on the heteronuclear variant introduced as SABRE-SHEATH (SABRE in SHield Enables Alignment Transfer to Heteronuclei) and nitrogen-15 targets in particular. We show that N-15-SABRE works more efficiently and on a wider range of substrates than H-1-SABRE, greatly generalizing the SABRE approach. In addition, we show that nitrogen-1s offers significantly extended T-1 times of up to 12 minutes. Long T-1 times enable higher hyperpolarization levels but also hold the promise of hyperpolarized molecular imaging for several tens of minutes. Detailed characterization and optimization are presented, leading to nitrogen-15 polarization'evels in excess of 10% on several compounds.