A TOF mass spectrometer for the study of noncovalent complexes
A TOF mass spectrometer for the study of noncovalent complexes
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DOI：
10.1016/j.ijms.2011.08.009
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发表时间：
2011-11-01
影响因子：
1.8
通讯作者：
Standing, K. G.
中科院分区：
文献类型：
--
作者：
Kozlovski, V. I.;Donald, L. J.;Standing, K. G.
描述了一种飞行时间（TOF）质谱仪，特别用于非共价复合物和大蛋白质的研究。在该装置中，加热的毛细管将电喷雾或纳米喷雾源耦合到仅RF四极离子导向器。离子导向器提供碰撞冷却，并将离子束注入TOF质谱仪。16 kV TOF加速电位提高了传输和检测效率，并且还增加了给定提取频率的m/z范围。两级反射器，加上离子导向器中的碰撞冷却，为小分子提供了高达18，000（FWHM）的分辨率，并为较大分子提供了一定程度的分辨率，尽管非共价峰的宽度主要由其固有的扩展决定。在我们的实验室用这种仪器获得的m/z光谱的例子。(C)2011 Elsevier B. V.保留所有权利。
A time-of-flight (TOF) mass spectrometer intended particularly for the study of noncovalent complexes and large proteins is described. In this device, a heated capillary couples an electrospray or nanospray source to an RF-only quadrupole ion guide. The ion guide provides collisional cooling, and injects the ion beam into a TOF mass spectrometer. 16 kV TOF acceleration potential improves transmission and detection efficiency, and also increases the m/z range for a given extraction frequency. A two-stage reflector, together with collisional cooling in the ion guide, provides resolving power up to 18,000 (FWHM) for small molecules, and somewhat improved resolution for larger ones, although the widths of the noncovalent peaks are mainly determined by their intrinsic spreads. Examples of m/z spectra obtained with this instrument in our laboratory are presented. (C) 2011 Elsevier B.V. All rights reserved.