Synthesis of Base-Modified 2′-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology
Synthesis of Base-Modified 2′-Deoxyribonucleoside Triphosphates and Their Use in Enzymatic Synthesis of Modified DNA for Applications in Bioanalysis and Chemical Biology
复制标题
DOI：
10.1021/jo5020799
复制
发表时间：
2014-11-07
影响因子：
3.6
通讯作者：
Hocek, Michal
中科院分区：
文献类型：
--
作者：
Hocek, Michal
讨论了通过核苷的经典三磷酸化或卤代dNTPs的水相交叉偶联反应合成2 '-脱氧核苷三磷酸（dNTPs）。综述了通过聚合酶掺入修饰的核苷酸合成修饰的寡核苷酸和DNA的不同酶促方法，并概述了它们在氧化还原或荧光标记以及在生物缀合和调节DNA与蛋白质相互作用中的应用。
The synthesis of 2'-deoxyribonucleoside triphosphates (dNTPs) either by classical triphosphorylation of nucleosides or by aqueous cross-coupling reactions of halogenated dNTPs is discussed. Different enzymatic methods for synthesis of modified oligonucleotides and DNA by polymerase incorporation of modified nucleotides are summarized, and the applications in redox or fluorescent labeling, as well as in bioconjugations and modulation of interactions of DNA with proteins, are outlined.