Urinary nickel as bioindicator of workers' Ni exposure in a galvanizing plant in Brazil
Urinary nickel as bioindicator of workers' Ni exposure in a galvanizing plant in Brazil
复制标题
DOI：
10.1007/pl00007940
复制
发表时间：
2000-01-01
影响因子：
3
通讯作者：
da Silva, CS
中科院分区：
文献类型：
--
作者：
Oliveira, JP;de Siqueira, MEPB;da Silva, CS
目的：我们测量了来自一家使用硫酸镍的镀锌厂的10名工人（97个样本）和10名对照受试者（55个样本）的尿镍（U-Ni），以研究职业暴露于空气中镍和镍吸收之间的关系。方法：连续5个工作日，在轮班前后抽取暴露组样本。同时用个人采样器测量了空气中Ni （A-NI）的含量。采用石墨炉原子吸收光谱法测定了生物材料和空气中镍的含量。对照组在轮班前和轮班后每天采集两次尿样。连续3天，结果：厂内所有检测场所均检测到低至中等水平的镍暴露，空气中镍暴露水平在2.8 ~ 116.7 μ g/m(3)之间，夜班尿样中镍暴露水平在3.5 ~ 43.2 μ g/g肌酐之间（平均13.7 μ g/g肌酐）。U-Ni肌酐在暴露组和对照组之间存在显著差异（Student’st检验，P <或等于0.01），U-Ni和A- ni之间存在显著相关性（r = 0.96, P <或等于0.001）。连续5天接触工人的U-Ni含量无统计学差异，但在轮班前和轮班后的样本之间存在显著差异。结论：尿镍可作为一种可靠的内剂量生物指标，用于镀锌厂暴露于硫酸镍的工人的生物监测，无论采集样品的工作日是哪一天。
Objectives: We measured urinary nickel (U-Ni) in ten workers (97 samples) from a galvanizing plant that uses nickel sulfate, and in ten control subjects (55 samples) to examine the association between occupational exposure to airborne Ni and Ni absorption.Methods: Samples from the exposed group were taken before and after the work shift on 5 successive workdays. At the same time airborne Ni (A-NI) was measured using personal samplers. Ni levels in biological material and in the airborne were determined by a graphite furnace atomic absorption spectrometry validated method. In the control group the urine samples were collected twice a day, in the before and after the work shift? on 3 successive days,Results: Ni exposure low to moderate was detected in all the examined places in the plant, the airborne levels varying between 2.8 and 116.7 mu g/m(3) and the urine levels, from samples taken postshift, between 3.5 and 43.2 mu g/g creatinine (mean 13.7 mu g/g creatinine). Significant differences in U-Ni creatinine were seen between the exposed and control groups (Student's t test, P less than or equal to 0.01), A significant correlation between U-Ni and A-Ni (r = 0.96; P less than or equal to 0.001) was detected. No statistical difference was observed in U-Ni collected from exposed workers in the 5 successive days, but significant difference was observed between pre- and postshift samples.Conclusions: Urinary nickel may be used as a reliable internal dose bioindicator in biological monitoring of workers exposed to Ni sulfate in galvanizing plants regardless of the day of the workweek on which the samples are collected.