Novel biodegradable three-dimensional macroporous scaffold using aligned electrospun nanofibrous yarns for bone tissue engineering
Novel biodegradable three-dimensional macroporous scaffold using aligned electrospun nanofibrous yarns for bone tissue engineering
复制标题
使用定向静电纺纳米纤维纱线用于骨组织工程的新型可生物降解三维大孔支架
DOI：
10.1002/jbm.a.34063
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发表时间：
2012-05-01
影响因子：
4.9
通讯作者：
Zou, Xiao-Hui
中科院分区：
文献类型：
--
作者：
Cai, You-Zhi;Zhang, Guo-Rong;Zou, Xiao-Hui
本研究旨在开发一种实用的三维(3D)大孔支架，用于骨组织工程。以电纺聚L-乳酸和聚己内酯(w/w 9：1)纳米纤维为原料，在65℃下通过连续纱线制造和蜂窝成型工艺制备了一种新型的三维未织造大孔纳米纤维支架。在体外，3DMNF支架有更多的细胞增殖和细胞内生长。HESC-MSCs在体外分化过程中可见明显的钙沉积。活体组织学和X线片显示，3DMNF支架处理后3周和6周，支架周围和支架内均有细小的3D骨组织形成。本研究表明，3DMNF支架为hESC-MSCs的生长提供了结构上的支持，并引导了骨的形成，提示这一新的策略成功地将电纺纤维用于骨组织工程，这可能有助于实现电纺纳米纤维在未来再生医学中的临床转化。(C)2012年威利期刊公司生物材料资源A部分：，2012年。
This study aimed to develop a practical three-dimensional (3D) macroporous scaffold from aligned electrospun nanofibrous yarns for bone tissue engineering. A novel 3D unwoven macroporous nanofibrous (MNF) scaffold was manufactured with electrospun poly(L-lactic acid) and polycaprolactone (w/w 9:1) nanofibers through sequential yarns manufacture and honeycombing process at 65 degrees C. The efficacy of 3D MNF scaffold for bone formation were evaluated using human embryonic stem cell-derived mesenchymal stem cells (hESC-MSCs) differentiation model and rabbit tibia bone defect model. In vitro, more cell proliferation and cell ingrowth were observed in 3D MNF scaffold. Moreover, calcium deposit was obviously detected in vitro differentiation of hESC-MSCs. In vivo, histology and X-ray showed that 3D MNF scaffold treated bone defect had fine 3D bony tissue formation around the scaffold as well as inside the scaffold at 3 weeks and 6 weeks. This study demonstrated that 3D MNF scaffold provides a structural support for hESC-MSCs growth and guides bone formation suggesting that this novel strategy successfully makes use of electrospun fibers for bone tissue engineering, which may help realize the clinical translation of electrospun nanofibers for regenerative medicine in future. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.