Reduction of p120ctn isoforms 1 and 3 is significantly associated with metastatic progression of human lung cancer
Reduction of p120ctn isoforms 1 and 3 is significantly associated with metastatic progression of human lung cancer
复制标题
p120ctn 亚型 1 和 3 的减少与人类肺癌的转移进展显着相关
DOI：
10.1111/j.1600-0463.2007.apm_673.x
复制
发表时间：
2007-07-01
期刊：
影响因子：
2.8
通讯作者：
Wang, En-Hua
中科院分区：
文献类型：
--
作者：
Liu, Yang;Xu, Hong-Tao;Wang, En-Hua
P120-catenin在细胞黏附和信号转导中发挥重要作用，但其亚型的功能尚不清楚。本研究的目的是检测p120-catenin亚型在肺癌中的表达，并探讨其与肺鳞癌和腺癌临床病理因素的关系。采用p120-catenin免疫荧光、Western印迹和逆转录-聚合酶链式反应(RT-PCR)检测p120-catenin在肺癌组织和肺癌细胞中的表达。P120-catenin在相应的正常支气管上皮细胞胞膜上可见清晰而连续的红色荧光，而在肺癌组织中未见p120-catenin胞膜表达减少或缺失或胞浆积聚。与相应的正常肺组织相比，肺癌组织中p120-catenin蛋白(P<；0.001)和m RNA的表达水平显著降低(P<；0.001)。P120-catenin在正常肺组织中表达的主要亚型是亚型1(120kD)和亚型3(100kD)，在肺癌组织中表达明显减少(P=0.001和P<；0.001)。P120-连环蛋白亚型在相应的正常肺组织中表达，而在肺癌组织中明显缺失(P<0.001和P=0.001)。此外，p120-连环蛋白亚型1与淋巴结转移呈负相关，而p120-连环蛋白亚型3与淋巴结转移呈正相关。我们得出结论，异构体1和3的减少可能在人类肺癌的转移进展中发挥不同的作用。
P120-catenin plays an important role in cell adhesion and signalling transduction though the function of its isoforms is unclear. The aim of this study was to examine the expression of p120-catenin isoforms in lung cancer and investigate their relationship to clinicopathological factors in lung squamous cell carcinomas (SCCs) and adenocarcinomas. The expression patterns of p120-catenin in lung cancer tissues and lung cancer cells were examined by p120-catenin immunofluorescence, Western blot, and reverse transcription-polymerase chain reaction (RT-PCR). Clear and continuous red fluorescence of p120-catenin is displayed at the cell membrane of corresponding normal bronchial epithelial cells, but not in lung cancer tissues that show reduction or absence of membrane expression of p120-catenin or cytoplasmic accumulation of p120-catenin. Compared with corresponding normal lung tissues, lung cancer tissues have significantly lower levels of p120-catenin proteins (P < 0.001) and mRNA (P < 0.001). The isoforms 1 (120 kD) and 3 (100 kD) proteins were major isoforms of p120-catenin expressed in normal lung tissues, which were significantly reduced in lung cancer samples (P=0.001 and P < 0.001, respectively). The mRNA of p120-catenin isoforms 1.2, 1.3, 2.3, 3.1 and 3.3 was detected in corresponding normal lung tissues, but was significantly absent in lung cancer samples (P < 0.001 and P=0.001, respectively). Furthermore, p120-catenin isoform 1 is negatively associated-whereas p120-catenin isoform 3 is positively associated-with lymph node metastasis. We conclude that reductions of isoforms 1 and 3 may play different roles in metastatic progression of human lung cancer.