Detection of circulating cancer cells in lung cancer patients with a panel of marker genes
Detection of circulating cancer cells in lung cancer patients with a panel of marker genes
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DOI：
10.1016/j.bbrc.2008.05.101
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发表时间：
2008-08-08
影响因子：
3.1
通讯作者：
Yin, Yan-hui
中科院分区：
文献类型：
--
作者：
Liu, Lei;Liao, Guo-qing;Yin, Yan-hui
目前的研究是使用一组标志物来检查肺癌患者的循环癌细胞，并评估这些测试的临床意义。从134例肺癌患者、106例良性肺部疾病患者和80例健康人的外周血单个核细胞（PBMCs）中分离并采用巢式逆转录- pcr方法检测三种不同肿瘤标志物的表达，包括肿瘤特异性抗原9 （TSA-9）、角蛋白19 （KRT-19）和前原胃泌素。释放肽（Pre-proGRP）。受试者工作特征曲线（Receiver operating characteristic curve， ROC）分析显示，联合使用这些标志物在鉴别癌症患者与健康和良性肺部疾病对照时具有高度的敏感性和特异性。在134例肺癌患者血液样本中，84.3%至少表达一种肿瘤标志物。阳性标记物的数量与疾病的分期和进展有显著的相关性。在非小细胞肺癌患者中，一个以上标记物的阳性预示着治疗反应差和生存时间短。(c) 2008爱思唯尔公司版权所有。
The current study was undertaken to examine the circulating cancer cells of lung cancer patients using a panel of markers and to evaluate the clinical significance of such tests. Peripheral blood mononuclear cells (PBMCs) from 134 lung cancer patients, 106 benign pulmonary disease, and 80 healthy individuals were isolated and assessed by nested reverse transcription-PCR assay for the expression of three different tumor markers, including tumor specific antigen 9 (TSA-9), Keratin 19 (KRT-19), and Pre-progastrin. releasing peptide (Pre-proGRP). Receiver operating characteristic curve (ROC) analysis showed that the combination of these markers was highly sensitive and specific in differentiating cancer patients from healthy and benign pulmonary disease controls. Of the 134 lung cancer patient blood samples, 84.3% expressed at least one tumor marker. A significant correlation was observed between the number of positive markers and disease stage and progression. Positivity of more than one marker predicted a poor response to therapy and short survival time in non-small cell lung cancer patients. (c) 2008 Elsevier Inc. All rights reserved.