Improvement of ACE inhibitory activity of chitooligosaccharides (COS) by carboxyl modification
Improvement of ACE inhibitory activity of chitooligosaccharides (COS) by carboxyl modification
复制标题
DOI：
10.1016/j.bmc.2005.03.034
复制
发表时间：
2005-06-01
影响因子：
3.5
通讯作者：
Kim, SK
中科院分区：
文献类型：
--
作者：
Huang, R;Mendis, E;Kim, SK
在本研究中，壳寡糖（COS）与- coch2ch2coo -基团羧基化，获得类似于卡托普利的特定结构特征（(R)）。研究了羧化COS对血管紧张素I转换酶（ACE）的抑制活性，发现其活性随着取代度的增加而增强。此外，Lineweaver-Burk图分析显示，通过酶的强制结合位点，抑制是竞争性的。同时，带正电的四化氨基被不同取代度的COS取代，这对ACE抑制有负面影响。(c) 2005 Elsevier Ltd版权所有。
In the present research, chitooligosaccharides (COS) were carboxylated with -COCH2CH2COO- groups to obtain specific structural features similar to Captopril((R)). Angiotensin I converting enzyme (ACE) inhibitory activity of carboxylated COS was studied and observed to enhance its activity with increased substitution degree. Further, Lineweaver-Burk plot analysis revealed that inhibition was competitive via obligatory binding site of the enzyme. This was accompanied with substitution of positively charged quarternized amino groups to COS with different substitution degrees, in which negative impact on ACE inhibition was observed. (c) 2005 Elsevier Ltd. All rights reserved.