Proteomics and lipidomics integrated analysis of outer membrane vesicles indicates that glycerophospholipid metabolism contributes to the early symbiosis between Sinorhizobium fredii HH103 and soybean.
Proteomics and lipidomics integrated analysis of outer membrane vesicles indicates that glycerophospholipid metabolism contributes to the early symbiosis between Sinorhizobium fredii HH103 and soybean.
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DOI：
10.1094/mpmi-11-21-0288-r
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发表时间：
2022-01
期刊：
影响因子：
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通讯作者：
Dongzhi Li;Ziqi Li;Jing Wu;Zhide Tang;F. Xie;Dasong Chen;Hui Lin;Youguo Li
中科院分区：
文献类型：
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作者：
Dongzhi Li;Ziqi Li;Jing Wu;Zhide Tang;F. Xie;Dasong Chen;Hui Lin;Youguo Li
革兰氏阴性菌能产生外膜囊泡（OMV），目前对OMV功能的研究主要集中在细菌与革兰氏阴性菌的相互作用上。然而，目前对根瘤菌OMV的研究还很有限。本研究以费氏中华根瘤菌（Sinorhizobium fredii HH 103）为材料，分别在自由生活条件（C-OMV）和染料木素诱导的模拟共生条件（G-OMV）下，分离纯化了OMV。G-OMV处理的大豆根系表现出明显的根毛变形。G-OMV显著诱导了与早期共生相关的促生长基因的表达，而抑制了大豆防御基因的表达。蛋白质组学分析共鉴定出93个差异蛋白，主要涉及核糖体合成、鞭毛装配、双组分系统、ABC转运蛋白、氧化磷酸化、氮代谢、群体感应、甘油磷脂代谢和肽聚糖合成等方面。脂质组学分析共鉴定出45种差异脂质。OMV蛋白质组和脂质组数据的相关性分析显示，甘油磷脂代谢是富集的KEGG代谢途径，磷脂酰丝氨酸脱羧酶在G-OMV中表达显著上调。酶联免疫吸附试验证实了甘油磷脂代谢途径中与共生有关的三种脂质的变化。我们的研究结果表明，甘油磷脂代谢有助于根瘤菌大豆共生通过OMV。
Gram-negative bacteria can produce outer membrane vesicles (OMVs), and most functional studies of OMVs have been focused on mammalian-bacterial interactions. However, research on the OMVs of rhizobia is still limited so far. In this work, we isolated and purified OMVs from Sinorhizobium fredii HH103 under free-living conditions that was set as control (C-OMVs) and symbiosis-mimicking conditions that was induced by genistein (G-OMVs). The soybean roots treated with G-OMVs displayed significant deformation of root hairs. G-OMVs significantly induced the expression of nodulation genes related to early symbiosis, while inhibited that of the defense genes of soybean. Proteomics analysis identified a total of 93 differential proteins between C-OMVs and G-OMVs, which are mainly associated with ribosome synthesis, flagellar assembly, two-component system, ABC transporters, oxidative phosphorylation, nitrogen metabolism, quorum sensing, glycerophospholipid metabolism and peptidoglycan biosynthesis. A total of 45 differential lipids were identified in lipidomics analysis. Correlation analysis of OMV proteome and lipidome data revealed that glycerophospholipid metabolism is the enriched KEGG metabolic pathway, and the expression of phosphatidylserine decarboxylase was significantly up-regulated in G-OMVs. The changes in three lipids related to symbiosis in the glycerophospholipid metabolism pathway were verified by ELISA. Our results indicate that glycerophospholipid metabolism contributes to rhizobia-soybean symbiosis via OMVs.