A FAMILY OF IMMUNOLOGICALLY RELATED TRANSCRIPTION FACTORS THAT INCLUDES MULTIPLE FORMS OF ATF AND AP-1
A FAMILY OF IMMUNOLOGICALLY RELATED TRANSCRIPTION FACTORS THAT INCLUDES MULTIPLE FORMS OF ATF AND AP-1
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DOI：
10.1101/gad.2.10.1216
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发表时间：
1988-10-01
影响因子：
10.5
通讯作者：
GREEN, MR
中科院分区：
文献类型：
--
作者：
HAI, T;LIU, F;GREEN, MR
ATF是一种细胞转录因子，参与多种腺病毒E1A和细胞cAMP诱导型启动子的调节。利用DNA亲和层析技术，我们纯化了ATF，发现一系列多肽以序列特异性方式共纯化。我们证明，这些多肽代表了一个家庭的蛋白质，是相关的DNA结合特异性和免疫交叉反应。该家族包括转录因子AP-1，其识别序列GTGAGTcAA与ATF共有序列GTGA_CGTCAA的不同之处在于缺少胞嘧啶残基。我们的研究结果进一步表明，有多种形式的ATF和AP-1。免疫交叉反应性和相关的DNA结合特异性表明，ATF和AP-1含有相似的氨基酸序列，可能来自一个共同的基因。
ATF is a cellular transcription factor involved in the regulation of multiple adenovirus E1A- and cellular cAMP-inducible promoters. Using DNA affinity chromatography, we have purified ATF and found that a series of polypeptides copurify in a sequence-specific manner. We demonstrate that these polypeptides represent a family of proteins that are related by DNA-binding specificity and by immunological cross-reactivity. This family includes the transcription factor AP-1, whose recognition sequence, GTGAGTcAA, differs from the ATF consensus, GTGA_CGTCAA, by the absence of a cytosine residue. Our results further indicate that there are multiple forms of both ATF and AP-1. The immunological cross-reactivity and related DNA-binding specificities suggest that ATF and AP-1 contain similar amino acid sequences and may have originated from a common gene.