THE LIGHT-CHAIN P-28 ASSOCIATES WITH A SUBSET OF INNER DYNEIN ARM HEAVY-CHAINS IN CHLAMYDOMONAS AXONEMES
THE LIGHT-CHAIN P-28 ASSOCIATES WITH A SUBSET OF INNER DYNEIN ARM HEAVY-CHAINS IN CHLAMYDOMONAS AXONEMES
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DOI：
10.1091/mbc.6.6.697
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发表时间：
1995-06-01
影响因子：
3.3
通讯作者：
PIPERNO, G
中科院分区：
文献类型：
--
作者：
LEDIZET, M;PIPERNO, G
我们发现衣藻轴突的I2和I3内动力蛋白臂重链被分解为两类：一类与蛋白p28相关，另一类与蛋白caltractin/centrin相关。我们已经确定了编码p28基因的核苷酸序列，p28是一个轻链，与肌动蛋白和caltractin/ centrin一起，与衣藻轴突内动力蛋白臂I2和I3相关。P28是一种新型蛋白，与内动力蛋白臂重链的一个子集具有亲和力，但与微管蛋白或肌动蛋白结合蛋白没有明显的显著同源性。p28特异性抗血清显示p28沿整个轴突存在。相同的抗血清共免疫沉淀p28，肌动蛋白和动力蛋白重链2'和2。相反，抗caltractin/centrin抗血清共免疫沉淀caltractin/centrin肌动蛋白和重链2,3和3'。与p28相关的动力蛋白重链2（称为2A）很可能与与caltractin/centrin结合的动力蛋白重链2（称为2B）是不同的多肽。由重链2B、肌动蛋白和caltractin/centrin组成的复合物通过暴露于Nonidet P-40优先被提取，并且在缺乏动力蛋白调节复合物组分1和2的突变体中缺失。
We show here that I2 and I3 inner dynein arm heavy chains of Chlamydomonas axonemes are resolved into two classes: one class associated with the protein p28 and the other associated with the protein caltractin/centrin. We have determined the nucleotide sequence of the gene encoding p28, a light chain that, together with actin and caltractin/ centrin, is associated with inner dynein arms I2 and I3 of Chlamydomonas axonemes. p28 is a novel protein with affinity for a subset of the inner dynein arm heavy chains, but with no apparent significant homologies to tubulin- or actin-binding proteins. An antiserum specific for p28 showed that p28 is present along the entire axoneme. The same antiserum coimmunoprecipitated p28, actin, and dynein heavy chains 2' and 2. In contrast, an anti-caltractin/centrin antiserum coimmunoprecipitated caltractin/centrin actin, and the heavy chains 2, 3, and 3'. It is likely that the dynein heavy chain 2 associated with p28, referred to as 2A, is a different polypeptide from dynein heavy chain 2 bound to caltractin/centrin, referred to as 2B. The complex formed by heavy chain 2B, actin, and caltractin/centrin is preferentially extracted by exposure to Nonidet P-40 and is missing in mutants lacking components 1 and 2 of the dynein regulatory complex.