Gene Transcriptional and Metabolic Profile Changes in Mimetic Aging Mice Induced by D-Galactose.
Gene Transcriptional and Metabolic Profile Changes in Mimetic Aging Mice Induced by D-Galactose.
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D-半乳糖诱导模拟衰老小鼠的基因转录和代谢谱变化
DOI：
10.1371/journal.pone.0132088
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Song WW
中科院分区：
文献类型：
--
作者：
Zhou YY;Ji XF;Fu JP;Zhu XJ;Li RH;Mu CK;Wang CL;Song WW
D-半乳糖注射已被证明可诱导小鼠的许多变化，这些变化代表加速衰老。该小鼠模型已广泛用于抗衰老剂的药理学研究。D-半乳糖致衰老的机制尚不清楚，但可能与糖脂代谢紊乱有关。目前，还没有一项研究集中在研究D-半乳糖衰老小鼠的基因表达变化。本研究采用基于气相色谱/质谱的代谢组学和基因表达谱的综合分析方法，研究了D-半乳糖注射拟衰老小鼠后转录和代谢谱的变化。我们的研究结果表明，48个mRNA在对照组和D-半乳糖小鼠之间差异表达，并在代谢水平上鉴定出51个潜在的生物标志物。D-半乳糖对衰老的影响可能与糖脂代谢紊乱、氧化损伤、糖基化终末产物（AGEs）积累、异常物质清除减少、细胞凋亡和胰岛素抵抗有关。
D-galactose injection has been shown to induce many changes in mice that represent accelerated aging. This mouse model has been widely used for pharmacological studies of anti-aging agents. The underlying mechanism of D-galactose induced aging remains unclear, however, it appears to relate to glucose and 1ipid metabolic disorders. Currently, there has yet to be a study that focuses on investigating gene expression changes in D-galactose aging mice. In this study, integrated analysis of gas chromatography/mass spectrometry-based metabonomics and gene expression profiles was used to investigate the changes in transcriptional and metabolic profiles in mimetic aging mice injected with D-galactose. Our findings demonstrated that 48 mRNAs were differentially expressed between control and D-galactose mice, and 51 potential biomarkers were identified at the metabolic level. The effects of D-galactose on aging could be attributed to glucose and 1ipid metabolic disorders, oxidative damage, accumulation of advanced glycation end products (AGEs), reduction in abnormal substance elimination, cell apoptosis, and insulin resistance.