Putative new species of the genus Dickeya as major soft rot pathogens in Phalaenopsis orchid production
Putative new species of the genus Dickeya as major soft rot pathogens in Phalaenopsis orchid production
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DOI：
10.1111/ppa.12677
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发表时间：
2017-10-01
期刊：
影响因子：
2.7
通讯作者：
Dreo, T.
中科院分区：
文献类型：
--
作者：
Alic, S.;Naglic, T.;Dreo, T.
细菌性软腐严重限制了兰花和其他园艺植物的生产。本文报道了从一个商业生产场所分离的有症状的蝴蝶兰致病菌的特征。经16S rDNA、fliC和dnaX部分测序，发现菌株间存在多样性，并将其分为两组，与此前报道的兰花中未定义的Dickeya谱系（UDL-3和UDL-4）最为相似。利用新一代测序技术对两个分离株（B16, S1）进行测序，为进一步研究提供了两个分离株的基因组草图（Ali等）。,)。新开发的基于fli的谱系特异性实时定量PCR检测用于区分谱系并评估其在病变组织中的相对丰度。对蝴蝶兰、马铃薯和菊苣叶片的体内毒力和侵袭性比较试验表明，与其他Dickeya属植物相比，这些新的Dickeya分离株具有高毒力和极强的浸渍潜力。此前报道的兰花软腐病病原体Pantoea cyprii（以前的Pectobacterium cyprii）未被检测到。人工感染蝴蝶兰后获得的分离株未引起症状。
Bacterial soft rots are a serious limitation to the production of orchids and other horticultural plants. Here, the characterization of causative bacteria isolated from Phalaenopsis orchids showing symptoms, from a commercial production site, is reported. The most commonly isolated bacteria were identified as Dickeya spp. Partial sequencing of 16S rDNA, fliC and dnaX showed diversity among the isolates and divided the isolates into two groups, with greatest similarity to previously reported undefined Dickeya lineages from orchids (UDL-3 and UDL-4). Two isolates (B16, S1) were sequenced using next-generation sequencing, which has provided draft genomes of these two isolates for further studies (Ali etal., ). Newly developed fliC-based lineage-specific quantitative real-time PCR assays were used to distinguish among the lineages and to assess their relative abundances in diseased tissues. Virulence and aggressiveness comparison tests invivo on Phalaenopsis orchids, potato plants and witloof chicory leaves indicated high virulence and extreme maceration potential of these novel Dickeya isolates, compared to a reference panel of other Dickeya spp. Pantoea cypripedii (formerly Pectobacterium cypripedii), which has previously been reported as a soft rot pathogen of orchids, was not detected, and isolates obtained from culture collections did not cause symptoms on artificially infected Phalaenopsis orchids.