Imprinting in neurons
Imprinting in neurons
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DOI：
10.1159/000090834
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发表时间：
2006-01-01
影响因子：
1.7
通讯作者：
Kishino, T.
中科院分区：
文献类型：
--
作者：
Kishino, T.
虽然大多数印迹基因在它们转录的组织中显示出亲本来源特异性基因表达，但一些基因以组织特异性方式印迹。显示脑特异性印记或脑特异性印记缺失的基因为研究组织分化过程中的印记过程提供了独特的机会。在这篇综述中，我介绍了使用原代脑细胞培养系统，其中神经元或胶质细胞分别培养的脑细胞谱系特异性印迹的系统研究。两份使用原代脑细胞培养的报告揭示了Ube 3a和Igf 2 r中的脑细胞谱系特异性印迹，这两种印迹先前分别被描述为显示脑特异性印迹和脑特异性印迹缺乏。这种脑细胞谱系特异性印迹与细胞特异性表观遗传修饰相关，特别是与其特异性印迹的反义非编码RNA、Ube 3a-ATS和Air相关。这些结果强调了在脑分化过程中，在细胞水平而不是在整个脑组织中进行印迹分析的必要性。脑细胞培养系统为我们了解脑特异性印迹的分子机制提供了一个新的有力工具。
Although most imprinted genes display parent-origin-specific gene expression in tissues where they are transcribed, some genes are imprinted in a tissue-specific manner. Genes that show brain-specific imprinting or brain-specific lack of imprinting present a unique opportunity to study the process of imprinting during tissue differentiation. In this review, I introduce the systematic study of brain-cell-lineage-specific imprinting using a primary brain cell culture system, where neurons or glial cells are cultured separately. Two reports using the primary brain cell culture revealed brain-cell-lineage-specific imprinting in Ube3a and Igf2r, which had previously been described to show brain-specific imprinting and brain-specific lack of imprinting, respectively. Such brain-cell-lineage-specific imprinting was associated with cell-specific epigenetic modifications, especially with their reciprocally imprinted antisense non-coding RNAs, Ube3a-ATS and Air. These results emphasize the necessity of imprinting analysis at the cell level rather than in whole brain tissue during brain differentiation. The brain cell culture system provides us with a new powerful tool to understand the molecular mechanism of brain-specific imprinting.