[Antimicrobial susceptibility profile of Acinetobacter baumannii complex isolates in Japan].
[Antimicrobial susceptibility profile of Acinetobacter baumannii complex isolates in Japan].
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[日本鲍曼不动杆菌复合株的抗菌敏感性概况]。
DOI：
10.11150/kansenshogakuzasshi.85.501
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
I. Kobayashi
中科院分区：
文献类型：
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作者：
F. Ikeda;Ayako Amano;Takako Iyoda;K. Matsuzaki;M. Hasegawa;T. Saika;A. Kanayama;I. Kobayashi
采用微量稀释法对2009年5 - 10月全国临床标本中分离的93株鲍曼不动杆菌复合菌进行药敏试验，并参照CLSI M100-S20标准进行药敏试验。使用PCR检测美罗培南非敏感株（包括中间或耐药株）中的β-内酰胺酶基因（包括B和D类以及ISAbal）。对美罗培南、环丙沙星和丁胺卡那霉素的不敏感率分别为18%、41%和14%。L7 ~ L 8：多重耐药不动杆菌属（MINE）对3种抗菌药物均耐药的比例为4.3%。多位点序列分型结果表明，Mp 60菌株属于ST 92型。在17株美罗培南非敏感菌株中未发现金属β-内酰胺酶产生菌。17株菌株均检出blaoxa-51样碳青霉烯酶基因和ISAbal。在17株分离株中的7株中观察到ISAba 1上游存在blaOXA-51样基因，在17株分离株中的5株中观察到blaOXA-23样基因。与海外报告一致，我们的结果证实了日本存在Mumbai分离株和携带OXA碳青霉烯酶基因的分离株。虽然耐药率低于其他地方的报告，但显然必须仔细监测耐药趋势。
The antimicrobial susceptibility of 93 Acinetobacter baumannii complex isolates from clinical specimens collected nationwide between May and October 2009 were measured by microdilution antimicrobial susceptibility testing based on CLSI M100-S20. Beta-lactamase genes, including classes B and D and ISAbal in meropenem nonsusceptible, including intermediate or resistant isolates, were detected using PCR. Rates of isolates nonsusceptible to meropenem were 18%, to ciprofloxacin 41% and to amikacin 14%. L7-L8: The rate of multidrug-resistant Acinetobacter (MDRA) isolates which were resistant to all 3 antimicrobial agents was 4.3%. MDRA isolates were classified into ST92 by multilocus sequence typing. No metallo-beta-lactamase producer was seen among the 17 meropenem nonsusceptible isolates. The blaoxa-51-like carbapenemase gene and ISAbal were detected in all 17 isolates. ISAba1 upstream presence of the blaOXA-51-like gene was observed in 7 of 17 isolates and the blaOXA-23 like gene in 5 of 17. Consistent with overseas reports, our results confirm the existence of MDRA isolates and isolates harboring OXA carbapenemase genes in Japan. While resistance rates were lower than reports elsewhere, it is clear that resistance trends must be carefully monitored.