Induction of macrophage-derived chemokine/CCL22 expression in experimental autoimmune encephalomyelitis and cultured microglia: implications for disease regulation
Induction of macrophage-derived chemokine/CCL22 expression in experimental autoimmune encephalomyelitis and cultured microglia: implications for disease regulation
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DOI：
10.1016/s0165-5728(02)00170-4
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发表时间：
2002-09-01
影响因子：
3.3
通讯作者：
Aloisi, F
中科院分区：
文献类型：
--
作者：
Columba-Cabezas, S;Serafini, B;Aloisi, F
巨噬细胞衍生趋化因子(MDC/CCL22)及其受体CCR4参与了慢性炎症过程以及单核细胞、Th2细胞和调节性T细胞亚群的归巢。在这里，我们证明了MDC和CCR4 mRNAs在发生复发缓解型和慢性复发型实验性自身免疫性脑脊髓炎(EAE)的小鼠的中枢神经系统(CNS)中表达。免疫组织化学显示，MDC由中枢神经系统浸润性白细胞和实质内小胶质细胞产生，而CCR4在一些侵袭性白细胞上表达。在体外激活后，小鼠小胶质细胞表达MDC转录并分泌生物活性MDC，诱导Th2细胞趋化，但不诱导Th1细胞趋化。我们认为，小胶质细胞产生的MDC可能通过促进Th2的归巢和可能的调节性T细胞进入病变部位来调节Th1介导的中枢神经系统炎症。(C)2002 Elsevier Science B.V.保留所有权利。
Macrophage-derived chemokine (MDC/CCL22) and its receptor CCR4 have been implicated in chronic inflammatory processes and in the homing of monocytes, Th2 cells and regulatory T-cell subsets. Here, we demonstrate that MDC and CCR4 mRNAs are expressed in the central nervous system (CNS) of mice developing relapsing-remitting and chronic-relapsing forms of experimental autoimmune encephalomyelitis (EAE). By immunohistochemistry, we show that MDC is produced by CNS-infiltrating leukocytes and intraparenchymal microglia, whereas CCR4 is expressed on some invading leukocytes. Upon in vitro activation, mouse microglia express MDC transcripts and secrete bioactive MDC that induces chemotaxis of Th2, but not Th1 cells. We suggest that MDC produced by microglia could regulate Th1 mediated CNS inflammation by facilitating the homing of Th2 and, possibly, regulatory T cells into the lesion site. (C) 2002 Elsevier Science B.V. All rights reserved.