A RNA.DNA hybrid that can adopt two conformations: an x-ray diffraction study of poly(rA).poly(dT) in concentrated solution or in fibers.
A RNA.DNA hybrid that can adopt two conformations: an x-ray diffraction study of poly(rA).poly(dT) in concentrated solution or in fibers.
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可以采用两种构象的 RNA.DNA 杂交体：浓缩溶液或纤维中的聚 (rA).聚 (dT) 的 X 射线衍射研究。
DOI：
10.1073/pnas.78.1.78
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发表时间：
1981
影响因子：
11.1
通讯作者：
B. H. Pheiffer
中科院分区：
文献类型：
--
作者：
S. Zimmerman;B. H. Pheiffer
RNA.DNA杂合聚(rA).聚(dT)可以采用两种构象，具体取决于其水合程度。纤维在 79% 相对湿度下产生传统的 A' RNA 样图案。相比之下，在高度溶剂化的条件下，这种材料产生的 X 射线衍射图与 B DNA 的 X 射线衍射图具有惊人的相似性，并且与之前从其他杂交纤维获得的类似 RNA 的衍射图明显不同。为混合体的解决方案形式提出了一个结构模型，该模型与为 B DNA 提出的模型有一些相似之处。该混合模型将聚（rA）链的2'-羟基容纳在与相邻核糖部分的链内氢键中。
The RNA.DNA hybrid poly(rA).poly(dT) can adopt two conformations, depending upon its degree of hydration. Fibers yield a conventional A' RNA-like pattern at 79% relative humidity. In contrast, under highly solvated conditions this material yields an x-ray diffraction pattern with striking similarities to that of B DNA and clearly different from the RNA-like diffraction patterns previously obtained from fibers of other hybrids. A structural model is proposed for the solution form of the hybrid that has several similarities to models proposed for B DNA. The hybrid model accommodates the 2'-hydroxyl groups of the poly(rA) strand in intrachain hydrogen bonds to the adjacent ribose moieties.