Visualization of primordial germ cells in vivo using GFP-nos1 3′UTR mRNA
Visualization of primordial germ cells in vivo using GFP-nos1 3′UTR mRNA
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DOI：
10.1387/ijdb.062143ts
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发表时间：
2006-01-01
影响因子：
0.7
通讯作者：
Yamaha, Etsuro
中科院分区：
文献类型：
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作者：
Saito, Taiju;Fujimoto, Takafumi;Yamaha, Etsuro
在一些硬骨鱼中，原始生殖细胞(PGCs)遗传特定的母体细胞质因子，如Vasat和Nanos 1(NOS1)mRNA。已有研究表明，VasA和NOS1的3‘非翻译区对斑马鱼PGC中这些RNA的稳定起着关键作用。在本研究中，我们将人工合成的结合绿色荧光蛋白(GFP)和斑马鱼NOS13‘UTR(GFP-NOS13’UTRmRNA)的mRNA注入不同物种的受精卵中，以确定这种作用是否在硬骨鱼之间保守。用同样的方法测定了水稻3‘端非编码区的同源基因(OLVAS)。我们证明了7种硬骨鱼的PGC可以用GFP-NOS13‘UTRmRNA进行可视化。GFP-OLVAS 3‘UTRmRNA不能在鲱鱼和泥鳅胚胎中检测到PGCs，但能够在青竹胚胎中显示PGCs。我们的结果表明，斑马鱼NOS1基因的3‘非编码区可以促进不同种类鱼类PGC中RNAs的维持。最后，我们描述并比较了七种硬骨鱼中PGC的迁移途径。
In some teleost fish, primordial germ cells (PGCs) inherit specific maternal cytoplasmic factors such as vasat and nanos 1 (nos1) mRNA. It has been shown that the 3' untranslated regions (UTRs) of vasa and nos1 have critical roles for stabilization of these RNAs in zebrafish PGCs. In this study, to determine whether this role of the nos1 3'UTR isconserved between teleost species,we injected artificially synthesized mRNA, combining green fluorescent protein (GFP) and the zebrafish nos1 3'UTR (GFP-nos1 3'UTR mRNA), into the fertilized eggs of various fish species. The 3'UTR of the Oryzias latipes vasa homologue (olvas) mRNA was assayed in the same manner. We demonstrate that the PGCs of seven teleost species could be visualized using GFP-nos1 3'UTR mRNA. GFP-olvas 3'UTR mRNA did not identify PGCs in herring or loach embryos, but did enable visualization of the PGCs in medaka embryos. Our results indicate that the 3'UTR of the zebrafish nos1 mRNA can promote maintenance of RNAs in the PGCs of different fish species. Finally, we describe and compare the migration routes of PGCs in seven teleost species.