Altered proteoglycan metabolism in mouse limb mesenchyme cell cultures treated with vitamin A.
Altered proteoglycan metabolism in mouse limb mesenchyme cell cultures treated with vitamin A.
复制标题
用维生素 A 处理的小鼠肢体间充质细胞培养物中蛋白多糖代谢发生改变。
DOI：
10.1016/0003-9861(78)90445-9
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发表时间：
1978
影响因子：
3.9
通讯作者：
J. Hassell
中科院分区：
文献类型：
--
作者：
J. P. Pennypacker;C. A. Lewis;J. Hassell
在小鼠肢体间充质培养物中检测了维生素 A 对软骨形成的抑制作用。对照培养物中的软骨形成的特征在于主要由硫酸软骨素组成的蛋白聚糖的合成增加。维生素 A 培养物中合成的蛋白聚糖比软骨中合成的蛋白聚糖小，并且发现主要含有硫酸乙酰肝素和硫酸皮肤素。没有观察到维生素 A 处理导致蛋白多糖降解增加的迹象。软骨形成前的对照培养物和维生素 A 培养物的蛋白聚糖在大小和糖胺聚糖组成上的相似性表明维生素 A 使细胞维持在间充质样状态。维生素 A 还刺激特定糖肽部分的甘露糖基化。
The inhibition of chondrogenesis by vitamin A was examined in mouse limb mesenchyme cultures. Chondrogenesis in control cultures was characterized by increased synthesis of proteoglycans composed predominantly of chondroitin sulfate. The proteoglycans synthesized in vitamin A cultures were smaller than those from cartilage and were found to contain mainly heparan sulfate and dermatan sulfate. No indication of increased proteoglycan degradation resulting from vitamin A treatment was observed. The similarity in size and glycosaminoglycan composition of proteoglycans from control cultures prior to chondrogenesis and vitamin A cultures suggests that vitamin A maintains the cells in a mesenchyme-like state. Vitamin A also stimulated the mannosylation of a specific fraction of glycopeptides.