A technique for the in vivo study of multiple stable isotope-labeled essential fatty acids
A technique for the in vivo study of multiple stable isotope-labeled essential fatty acids
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DOI：
10.1054/plef.2002.0411
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发表时间：
2002-08-01
影响因子：
3
通讯作者：
Salem, N
中科院分区：
文献类型：
--
作者：
Lin, Y;Salem, N
提出了一种同时独立测量多种稳定同位素标记必需脂肪酸的新型示踪技术。采用气相色谱/负化学电离质谱法对以下同位素进行高灵敏度检测：氘标记的亚麻酸盐、碳-13- u标记的二十碳五烯酸盐、碳-13- u标记的亚油酸盐和氘标记的二同γ -亚麻酸盐。这些同位素标记的脂肪酸在车用油中单独或一起给予大鼠单次口服剂量。给药后采集大鼠血液，同时检测前体及其主要代谢物的同位素，包括同时含有C-13和H-2的同位素，具有良好的分辨率，并且由于质量和色谱保留的差异，没有受到其他同位素的干扰。Elsevier Science Ltd.出版。
A novel tracer technique is presented for the simultaneous and independent measurement of multiple stable isotopically labeled essential fatty acids. Gas chromatography/negative chemical ionization mass spectrometry was employed for high sensitivity detection of the following isotopes: deuterium-labeled-linolenate, carbon-13-U-labeled-eicosapentaenoate, carbon-13-U-labeled-linoleate, and deuterium-labeled-dihomo-gamma-linolenate. These isotope-labeled fatty acids in vehicle oil were given to rats either singly or together as a single oral dose. Rat blood was collected after dosing and the isotopomers of the precursors and their main metabolites, including those containing both C-13 and H-2, were detected simultaneously with good resolution and without interference from other isotopes due to differences in mass and chromatographic retention. Published by Elsevier Science Ltd.