Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells.
Isoorientin induces apoptosis, decreases invasiveness, and downregulates VEGF secretion by activating AMPK signaling in pancreatic cancer cells.
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异荭草素通过激活胰腺癌细胞中的 AMPK 信号传导诱导细胞凋亡、降低侵袭性并下调 VEGF 分泌
DOI：
10.2147/ott.s122653
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发表时间：
2016
影响因子：
4
通讯作者：
Zhou M
中科院分区：
文献类型：
--
作者：
Ye T;Su J;Huang C;Yu D;Dai S;Huang X;Chen B;Zhou M
异orientin（或homoorientin）是一种黄酮，是一种化学类黄酮化合物，也是木犀草素的6-C-葡萄糖苷。异orientin已被证明对多种肿瘤具有抗癌活性，但其对胰腺癌（PC）的作用尚未详细研究。本研究旨在探讨异东方草素是否具有潜在的抗PC作用及其机制。在PC中，异芫荽素强烈抑制细胞存活，诱导细胞凋亡，并通过逆转上皮-间充质转化和基质金属蛋白酶的表达以及降低血管内皮生长因子的表达来降低其恶性程度。与此同时，我们研究了AMP激活的蛋白激酶（AMPK）信号通路的活性后，异orientin处理，这是有力的激活异orientin，如预期的。此外，在用慢病毒转染以干扰基因PRKAA 1表达的PC细胞中，异orientin处理组和未处理组的肿瘤中的细胞凋亡率和恶性肿瘤生物标志物的表达没有差异。因此，我们证明了异orientin通过AMPK信号通路具有潜在的抗肿瘤作用，并且异orientin值得进一步研究。
Isoorientin (or homoorientin) is a flavone, which is a chemical flavonoid-like compound, and a 6-C-glucoside of luteolin. Isoorientin has been demonstrated to have anti-cancer activities against various tumors, but its effects on pancreatic cancer (PC) have not been studied in detail. In this study, we aim to investigate whether isoorientin has potential anti-PC effects and its underlying mechanism. In PC, isoorientin strongly inhibited the survival of the cells, induced cell apoptosis, and decreased its malignancy by reversing the expression of epithelial–mesenchymal transition and matrix metalloproteinase and decreased vascular endothelial growth factor expression. Meanwhile, we investigated the activity of the AMP-activated protein kinase (AMPK) signaling pathway after isoorientin treatment, which was forcefully activated by isoorientin, as expected. In addition, in the PC cells that were transfected with lentivirus to interfere with the expression of the gene PRKAA1, there were no differences in the apoptosis rate and the expression of malignancy biomarkers in the tumors of the isoorientin-treated and untreated groups. Thus, we demonstrated that isoorientin has potential antitumor effects via the AMPK signaling pathway, and isoorientin merits further investigation.