The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2- loaded hollow hydroxyapatite microspheres/chitosan composite
The mechanism research of non-Smad dependent TAK1 signaling pathway in the treatment of bone defects by recombination BMP-2- loaded hollow hydroxyapatite microspheres/chitosan composite
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DOI：
10.1007/s10856-019
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
熊龙
中科院分区：
文献类型：
--
作者：
李经堂;熊世烺;丁凌华;曾建华;邱鹏;周建国;廖新根;熊龙
Abstract.Aims The present study aimed to evaluate whether the non-Smad dependent TAK1 signaling pathway (BMP-2-TAK1-p38-.Osx signaling pathway) played an important role in bone repair mediated by hollow hydroxyapatite (HA) microspheres/.chitosan (CS) composite..Methods Firstly, the biological activity of rhBMP-2 released from the complex was investigated. Then, differentiation test.of osteoblasts including ALP activity and calcium deposition, X-ray scoring and three-point bending test were performed..Finally, the mRNAs expression of TAK1, p38, Osx and osteogenic markers was tested by reverse transcription-polymerase.chain reaction (RT-PCR)..Results RhBMP-2 could be loaded and released from the complex in bioactive form. Additionally, the complex provided a.prolonged period of time compared with HA/CS scaffolds. Serum ALP activity was significantly decreased in the TAK1.inhibitor group and p38 inhibitor group. In the X-ray radiography, bone callus was observed in rhBMP-2-loaded hollow HA.microspheres/CS composite group. In the three-point bending test, load values in p38 inhibitor group decreased. In the.animal model, the mRNA expression of BSP on day 90 was significantly decreased in the p38 inhibitor group and TAK1.inhibitor group. In MC3T3-E1 cells, the mRNA expression of OSX was remarkably up-regulated in both rhBMP-2 group or.rhBMP-2-loaded hollow HA microspheres/CS composite group; while the mRNA expression of OSX was significantly.down-regulated in TAK1 inhibitor group and p38 inhibitor group..Conclusion The BMP-2-TAK1-p38-OSX signaling pathway may play an important role in bone formation and repair.mediated by rhBMP-2-loaded hollow HA microspheres/CS composite.
