The complete mitochondrial genome of a turbinid vetigastropod from MiSeq Illumina sequencing of genomic DNA and steps towards a resolved gastropod phylogeny
The complete mitochondrial genome of a turbinid vetigastropod from MiSeq Illumina sequencing of genomic DNA and steps towards a resolved gastropod phylogeny
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DOI：
10.1016/j.gene.2013.10.005
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发表时间：
2014-01-01
期刊：
影响因子：
3.5
通讯作者：
Littlewood, D. T. J.
中科院分区：
文献类型：
--
作者：
Williams, S. T.;Foster, P. G.;Littlewood, D. T. J.
需要增加对腹足动物线粒体基因组的采样已被确定为解决腹足动物内部关系的重要一步。我们使用Illumina MiSeq对基因组DNA进行了鸟枪测序，获得了vetigastropod family Turbinidae的第一个线粒体基因组，使物种丰富的超家族Trochoidea的基因组数量增加了一倍。该方法避免了为长pcr或引物行走扩增子寻找合适引物的必要性，从而为从乙醇保存的组织样本中获得全线粒体基因组提供了一种及时且经济有效的方法。对所有可用的腹足动物基因组（包括新的涡轮虫mtgenome）的氨基酸变异进行贝叶斯分析，得到了一个具有高节点支持度的高分辨率树。腹足纲中除Littorinimorpha属多系外，其余主要分支均得到了强有力的支持。我们在此确认，有丝分裂基因组学是软体动物系统发育的有用工具，特别是当使用强大的氨基酸进化新模型时，但认识到仍然需要增加分类群采样来解决核和线粒体基因树之间存在的差异。爱思唯尔B.V.版权所有
A need to increase sampling of mitochondrial genomes for Vetigastropoda has been identified as an important step towards resolving relationships within the Gastropoda. We used shotgun sequencing of genomic DNA, using an Illumina MiSeq, to obtain the first mitochondrial genome for the vetigastropod family Turbinidae, doubling the number of genomes for the species-rich superfamily Trochoidea. This method avoids the necessity of finding suitable primers for long PCRs or primer-walking amplicons, resulting in a timely and cost-effective method for obtaining whole mitochondrial genomes from ethanol-preserved tissue samples. Bayesian analysis of amino acid variation for all available gastropod genomes including the new turbinid mtgenome produced a well resolved tree with high nodal support for most nodes. Major clades within Gastropoda were recovered with strong support, with the exception of Littorinimorpha, which was polyphyletic. We confirm here that mitogenomics is a useful tool for molluscan phylogenetics, especially when using powerful new models of amino acid evolution, but recognise that increased taxon sampling is still required to resolve existing differences between nuclear and mitochondrial gene trees. Crown Copyright (C) 2013 Published by Elsevier B.V. All rights reserved.