Quantitative analysis of mammalian chromosome by scanning transmission soft X-ray microscopy
Quantitative analysis of mammalian chromosome by scanning transmission soft X-ray microscopy
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DOI：
10.1016/j.ultramic.2018.07.001
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发表时间：
2018-11-01
期刊：
影响因子：
2.2
通讯作者：
Ito, A.
中科院分区：
文献类型：
--
作者：
Shinohara, K.;Ohigashi, T.;Ito, A.
应用软 X 射线光谱显微镜以 100 nm 的空间分辨率研究哺乳动物染色体中 DNA 和蛋白质的定量分布。 DNA 和蛋白质的量使用 NEXAFS 光谱中氮 K 吸收边的 1s-pi* 跃迁进行评估。 DNA在染色体上分布不均匀，DNA/蛋白质比值小于0.497。目前的分析揭示了识别对样品吸收光谱有贡献的其他分子的线索。结果表明，相关分子吸收光谱的积累将支持分析的完善。
Soft X-ray spectromicroscopy was applied to study the quantitative distribution of DNA and protein in a mammalian chromosome at the spatial resolution of 100 nm. The quantities of DNA and protein were evaluated using 1s-pi* transition in the NEXAFS spectra at the nitrogen K absorption edge. DNA was not uniformly distributed in the chromosome and DNA/protein ratio was less than 0.497. The present analysis revealed the clues to identify other molecules that contribute to the absorption spectrum of the sample. The results suggested that accumulation of the absorption spectra of relevant molecules would support the refinement of the analysis.