Cell orientation determines the alignment of cell-produced collagenous matrix
Cell orientation determines the alignment of cell-produced collagenous matrix
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DOI：
10.1016/s0021-9290(02)00233-6
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发表时间：
2003-01-01
影响因子：
2.4
通讯作者：
Woo, SLY
中科院分区：
文献类型：
--
作者：
Wang, JHC;Jia, FY;Woo, SLY
在愈合韧带和肌腱时，细胞不对齐，胶原蛋白基质也不像正常组织那样组织起来。此外，组织的机械性能异常。我们假设胶原蛋白基质缺乏排列是由于愈合区域中细胞的随机方向造成的。为了检验这一假设，使用了一种新型体外模型，其中可以通过微槽控制细胞的方向，并且可以轻松观察到这些细胞形成的胶原蛋白基质的排列。众所周知，细胞沿着微纹方向均匀排列；因此，产生大量胶原蛋白的 MC3T3-E1 细胞在表面具有平行微槽（10 微米宽 x 3 微米深）的硅胶膜上生长。作为对照，相同的细胞也在光滑的硅胶膜上生长。微槽和光滑的硅胶表面上的细胞产生了一层清晰可见的胶原蛋白基质。免疫组织化学染色显示基质由丰富的 I 型胶原组成。胶原基质的偏光显微镜显示胶原纤维与微纹方向平行，而光滑膜上随机取向的细胞产生的胶原基质是杂乱的。因此，本研究的结果表明细胞的方向影响细胞产生的胶原基质的组织。结果还表明，沿着愈合韧带和肌腱的纵向定向细胞可能会导致产生更接近未受伤状态的对齐胶原基质。这可以增强愈合韧带和肌腱的机械性能。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
In healing ligaments and tendons, the cells are not aligned and collagen matrix is not organized as in normal tissues. In addition, the mechanical properties of the tissues are abnormal. We hypothesized that the lack of alignment of the collagen matrix results from random orientation of the cells seen in the healing area. To test this hypothesis, a novel in vitro model was used in which the orientation of cells could be controlled via microgrooves, and alignment of the collagen matrix formed by these cells could be easily observed. It is known that cells align uniformly along the direction of microgrooves; therefore MC3T3-E1 cells, which produce large amounts of collagen, were grown on silicone membranes with parallel microgrooves (10 mum wide x 3 mum deep) in the surface. As a control, the same cells were also grown on smooth silicone membranes. Cells on both the microgrooved and smooth silicone surfaces produced a layer of readily visible collagen matrix. Immunohistochemical staining showed that the matrix consisted of abundant type I collagen. Polarized light microscopy of the collagen matrix revealed the collagen fibers to be parallel to the direction of the microgrooves, whereas the collagen matrix produced by the randomly oriented cells on the smooth membranes was disorganized. Thus, the results of this study suggest that the orientation of cells affects the organization of the collagenous matrix produced by the cells. The results also suggest that orienting cells along the longitudinal direction of healing ligaments and tendons may lead to the production of aligned collagenous matrix that more closely represents the uninjured state. This may enhance the mechanical properties of healing ligaments and tendons. (C) 2002 Elsevier Science Ltd. All rights reserved.