RADIATION-DAMAGE RELATIVE TO TRANSMISSION ELECTRON-MICROSCOPY OF BIOLOGICAL SPECIMENS AT LOW-TEMPERATURE - REVIEW
RADIATION-DAMAGE RELATIVE TO TRANSMISSION ELECTRON-MICROSCOPY OF BIOLOGICAL SPECIMENS AT LOW-TEMPERATURE - REVIEW
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DOI：
10.1111/j.1365-2818.1978.tb01160.x
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发表时间：
1978-01-01
影响因子：
2
通讯作者：
TAYLOR, KA
中科院分区：
文献类型：
--
作者：
GLAESER, RM;TAYLOR, KA
当生物样品在EM中受到电子束照射时，由于分子激发、电离和随后的化学反应，样品结构被破坏。在正常的EM过程中发生的辐射损伤严重限制了生物标本的高分辨率成像细节。研究了低温辐射损伤的问题，以降低损伤发生的速度。对小分子（无水）有机化合物的辐射损伤防护通常相当有限，甚至不存在。与室温下的损伤率相比，大分子水合材料在低温下的辐射损伤发生率降低了10倍。因此，在水化标本的情况下，低温EM提供了一个重要的优势，作为获得复杂生物结构的高分辨率图像所需的整体努力的一部分。
When biological specimens are irradiated by the electron beam in the EM, the specimen structure is damaged as a result of molecular excitation, ionization and subsequent chemical reactions. The radiation damage that occurs in the normal process of EM presents severe limitations for imaging high resolution detail in biological specimens. The question of radiation damage at low temperatures was investigated to reduce the rate at which damage occurs. The radiation damage protection found for small molecule (anhydrous) organic compounds was generally rather limited or even non-existent. Large molecule, hydrated materials showed as much as a 10-fold reduction at low temperature in the rate at which radiation damage occurred, relative to the damage rate at room temperature. In the case of hydrated specimens, therefore, low temperature EM offered an important advantage as part of the overall effort required in obtaining high resolution images of complex biological structures.