The mer operon of a mercury-resistant Pseudoalteromonas haloplanktis strain isolated from Minamata Bay, Japan

The mer operon of a mercury-resistant Pseudoalteromonas haloplanktis strain isolated from Minamata Bay, Japan
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DOI:
10.1007/s002530100734
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发表时间:
2001-09-01
影响因子:
5
通讯作者:
Furukawa, K
Furukawa, K
中科院分区:
工程技术2区
文献类型:
--
作者:
Iohara, K;Iiyama, R;Furukawa, K

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对从水手湾海水中分离到的耐汞嗜盐假单胞菌M1菌株的1个操纵子进行了克隆和分析。Mer基因位于染色体上,排列顺序为MERR-MERT-MERP-MERC-MERA-MERD,顺序与Tn21相同。然而。MerR基因的方向与其他mer基因的方向相同(与TN21相反),MerR与其他mer基因共转录,这是以前在其他革兰氏阴性菌的mer决定簇中未曾见过的模式。此外,M1株和TN21株相应的mer基因产物的氨基酸相似性很低。
A mer operon of mercury-resistant Pseudoalteromonas haloplanktis strain M1, isolated from sea water of Minamata Bay, was cloned and analyzed. The mer genes were located in the chromosome an organized as merR-merT-merP-merC-merA-merD, the same order as that in Tn21. However. the orientation of the merR gene is the same as that of other mer genes (opposite direction to Tn21), and merR was cotranscribed with other mer genes, a pattern that has not been previously seen with mer determinants from other Gram-negative bacteria. Furthermore, the amino acid similarities of the corresponding mer gene products between those from strain M1 and Tn21 were unusually low.