Responses to noxious stimuli in mice lacking α1d-adrenergic receptors
Responses to noxious stimuli in mice lacking α1d-adrenergic receptors
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DOI：
10.1097/00001756-200310060-00020
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发表时间：
2003-10-06
期刊：
影响因子：
1.7
通讯作者：
Takano, Y
中科院分区：
文献类型：
--
作者：
Harasawa, I;Honda, K;Takano, Y
用甩尾法、热板法(舔后爪法和跳跃法)、夹尾法和福尔马林法观察了缺乏α(LD)-肾上腺素能受体(α(LD)-AR)小鼠和野生型小鼠的伤害性行为。用原位杂交法研究了α(LD)-AR在野生型小鼠体内的分布，发现突变小鼠的甩尾和舔后爪潜伏期较长，而跳跃潜伏期较短。在阿尔法(LD)基因敲除的小鼠中，机械和化学伤害性感受没有改变。原位杂交显示在丘脑网状核、海马区、扣带回皮质和脊髓有密集的α(Ld)-AR基因表达。这些结果表明，脊髓中的阿尔法(Ld)-AR参与了热原痛觉；当逃避热时，跳跃行为通过脊髓上的阿尔法(Ld)-AR被抑制。
Nociceptive behaviors were examined in the mice lacking alpha(ld)-adrenergic receptor ( alpha(ld)-AR) and wild type littermates using tail-flick, hot-plate (hindpaw-licking and jumping), tail-pinch and formalin tests. The distribution Of alpha(ld)-AR was studied using in situ hybridization in the wild type mice, Mutant mice showed longer tail-flick and hindpaw-licking latencies while their jumping latency was shorter. Mechanical and chemical nociception was not altered in alpha(ld)-knockout mice. In situ hybridization study revealed dense alpha(ld)-AR mRNA expression in the reticular thalamic nucleus, the hippocampus, the cingulate cortex and the spinal cord. These results suggest that alpha(ld)-AR in the spinal cord contributes to thermal pronociception; and that the jump behavior seen when escaping from heat is inhibited via the supraspinal alpha(ld)-AR.